English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 45073/58249 (77%)
造訪人次 : 2439340      線上人數 : 191
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋


    請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/60522


    題名: 結合表沒食子兒茶素-3-沒食子酸酯之中孔生物活性玻璃對抗菌能力與骨增生之體外評估
    In vitro evaluation of epigallocatechin-3-gallate with mesoporous bioactive glasses for antibacterial ability and bone regeneration
    作者: 王翊庭
    WANG, YI-TING
    貢獻者: 牙體技術學系碩士班
    范家瑜
    關鍵詞: 中孔生物玻璃支架;聚合物海綿複製法;EGCG;抗菌;藥物釋放
    mesoporous bioglass scaffold;foam exchange technique;epigallocatechin-3-gallate;antibacterial;drug release
    日期: 2020-12-22
    上傳時間: 2021-03-19 11:17:30 (UTC+8)
    摘要: 生物活性玻璃材料已被廣泛使用於生物醫學領域，本研究以聚合物海綿複製技術合成中孔生物活性玻璃(Mesoporous bioglass, MBG)作為載體，結合表沒食子兒茶素-3-沒食子酸酯(Epigallocatechin-3-gallate, EGCG)進行藥物傳遞系統評估。經由掃描式顯微鏡觀察到此複製法可形成一相互連接且無裂縫的孔洞支架結構，利用瓊脂稀釋法檢測出EGCG對金黃色葡萄球菌和大腸桿菌的最小抑菌濃度分別為25 μg/mL和200 μg/mL。將中孔生物活性玻璃浸泡200 μg/mL濃度製成EGCG-MBG支架，分別將之浸泡仿生溶液 (simulated body fluid, SBF)中以進行生物活性表現觀察，發現MBG與EGCG-MBG組別都具有良好的磷灰石礦化。EGCG-MBG的累積藥物釋放率為75%，且經過細胞毒性測試發現添加200 μg/ mL EGCG結合生物活性玻璃支架可以防止細胞凋亡並促進細胞增殖。因此，EGCG-MBG生物玻璃支架將提供未來骨缺損修復的另一種選擇。
    Mesoporous bioglass (MBG) materials have been widely used in the field of biomedicine. In this study, a polymeric sponge replication technique was applied to synthesize mesoporous bioglass (MBG) as the carrier. Epigallocatechin-3-gallate (EGCG) was jointly used to evaluate the drug delivery systems (DDS). Through the Scanning Electron Microscope (SEM), it was observed that this replication method contributed to the formation of an interconnected porous supporting structure without cracks. Through the agar dilution method, it was detected that the minimum inhibitory concentrations (MICs) of EGCG against E. coli and S. aureus were 200 μg/mL and 25 μg/mL, respectively. Mesoporous bioglass (MBG) was immersed in EGCG at a concentration of 200 μg/mL to produce EGCG-MBG scaffolds. They were each immersed in simulated body fluid (SBF) to observe bioactivity performance. It was found that both MBG and EGCG-MBG groups demonstrated excellent apatite mineralization. The cumulative drug release rate of EGCG-MBG was 75%. After a cytotoxicity test, it was found that the addition of 200μg/ mL EGCG combined with BMG scaffolds helped prevent cell apoptosis and promoted cell proliferation. Therefore, EGCG-MBG scaffolds shall serve as an alternative choice for the repair of bone defects in the future.
    描述: 碩士
    指導教授:范家瑜
    委員:林茂欽
    委員:沈永康
    資料類型: thesis
    顯示於類別:[牙體技術學系] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML232檢視/開啟


    檢視Licence

    在TMUIR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    著作權聲明 Copyright Notice

    • 本平台之數位內容為臺北醫學大學所收錄之機構典藏，包含體系內各式學術著作及學術產出。秉持開放取用的精神，提供使用者進行資料檢索、下載與取用，惟仍請適度、合理地於合法範圍內使用本平台之內容，以尊重著作權人之權益。商業上之利用，請先取得著作權人之授權。

      The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.

    • 瀏覽或使用本平台，視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例，並不得為任何不法目的使用TMUIR。

      By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.

    • 本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者，煩請權利人通知本平台維護人員([email protected])，將立即採取移除該數位著作等補救措施。

      TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

    Back to Top
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋