Taipei Medical University Institutional Repository:Item 987654321/60522
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    题名: 結合表沒食子兒茶素-3-沒食子酸酯之中孔生物活性玻璃對抗菌能力與骨增生之體外評估
    In vitro evaluation of epigallocatechin-3-gallate with mesoporous bioactive glasses for antibacterial ability and bone regeneration
    作者: 王翊庭
    WANG, YI-TING
    贡献者: 牙體技術學系碩士班
    范家瑜
    关键词: 中孔生物玻璃支架;聚合物海綿複製法;EGCG;抗菌;藥物釋放
    mesoporous bioglass scaffold;foam exchange technique;epigallocatechin-3-gallate;antibacterial;drug release
    日期: 2020-12-22
    上传时间: 2021-03-19 11:17:30 (UTC+8)
    摘要: 生物活性玻璃材料已被廣泛使用於生物醫學領域，本研究以聚合物海綿複製技術合成中孔生物活性玻璃(Mesoporous bioglass, MBG)作為載體，結合表沒食子兒茶素-3-沒食子酸酯(Epigallocatechin-3-gallate, EGCG)進行藥物傳遞系統評估。經由掃描式顯微鏡觀察到此複製法可形成一相互連接且無裂縫的孔洞支架結構，利用瓊脂稀釋法檢測出EGCG對金黃色葡萄球菌和大腸桿菌的最小抑菌濃度分別為25 μg/mL和200 μg/mL。將中孔生物活性玻璃浸泡200 μg/mL濃度製成EGCG-MBG支架，分別將之浸泡仿生溶液 (simulated body fluid, SBF)中以進行生物活性表現觀察，發現MBG與EGCG-MBG組別都具有良好的磷灰石礦化。EGCG-MBG的累積藥物釋放率為75%，且經過細胞毒性測試發現添加200 μg/ mL EGCG結合生物活性玻璃支架可以防止細胞凋亡並促進細胞增殖。因此，EGCG-MBG生物玻璃支架將提供未來骨缺損修復的另一種選擇。
    Mesoporous bioglass (MBG) materials have been widely used in the field of biomedicine. In this study, a polymeric sponge replication technique was applied to synthesize mesoporous bioglass (MBG) as the carrier. Epigallocatechin-3-gallate (EGCG) was jointly used to evaluate the drug delivery systems (DDS). Through the Scanning Electron Microscope (SEM), it was observed that this replication method contributed to the formation of an interconnected porous supporting structure without cracks. Through the agar dilution method, it was detected that the minimum inhibitory concentrations (MICs) of EGCG against E. coli and S. aureus were 200 μg/mL and 25 μg/mL, respectively. Mesoporous bioglass (MBG) was immersed in EGCG at a concentration of 200 μg/mL to produce EGCG-MBG scaffolds. They were each immersed in simulated body fluid (SBF) to observe bioactivity performance. It was found that both MBG and EGCG-MBG groups demonstrated excellent apatite mineralization. The cumulative drug release rate of EGCG-MBG was 75%. After a cytotoxicity test, it was found that the addition of 200μg/ mL EGCG combined with BMG scaffolds helped prevent cell apoptosis and promoted cell proliferation. Therefore, EGCG-MBG scaffolds shall serve as an alternative choice for the repair of bone defects in the future.
    描述: 碩士
    指導教授:范家瑜
    委員:林茂欽
    委員:沈永康
    数据类型: thesis
    显示于类别:[牙體技術學系] 博碩士論文

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