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http://libir.tmu.edu.tw/handle/987654321/9088
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| 題名: | Cholesterol-3-beta, 5-alpha, 6-beta,-triol induced genotoxicity through reactive oxygen species formation |
| 作者: | 鄭幼文
Cheng YW;Knag JJ;Shih YL;LO YL;Wang CF |
| 貢獻者: | 藥學系 |
| 日期: | 2005 |
| 上傳時間: | 2009-10-07 15:06:59 (UTC+8) |
| 摘要: | The mutagenicity of oxysterols, cholesterol-3beta,5alpha,6beta-triol (alpha-Triol), 7-keto-cholesterol (7-Keto) and cholesterol-5alpha,6alpha-epoxide (alpha-Epox) were examined by the Ames method and chromosome aberration test in this study. Only alpha-Triol concentration-dependently caused an increase of bacterial revertants in the absence of metabolic activating enzymes (S9), but not 7-keto and alpha-Epox. The mutagenic effect of alpha-Triol was reduced by the addition of S9. On the other hand, although alpha-Triol significantly induced chromosome aberration in CHO-K1 cells with and without S9. However, the addition of S9 reduced the degree of abnormal structure chromosome compared to without S9 mix. Catalase and superoxide dismutase (SOD) inhibited alpha-Triol induced increase of revertants in Salmonella typhimurium and chromosome aberration frequency in CHO cells, suggesting that reactive oxygen species (ROS) might be involved in the genotoxic effect of alpha-Triol. Treatment with alpha-Triol increased the ROS production in CHO cells, which could be attenuated by catalase and SOD. Results in this study suggested, for the first time that alpha-Triol, causes genotoxic effect in an ROS-dependent manner. |
| 關聯: | Food and Chemical Toxicology.(43):617-622. |
| 資料類型: | article |
| 顯示於類別: | [藥學系] 期刊論文
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