Taipei Medical University Institutional Repository:Item 987654321/6832
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 45069/58245 (77%)
造访人次 : 2370097      在线人数 : 178
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/6832


    题名: L-3-羥基丁酸於H9c2細胞株及大鼠組織內含量之分析
    The Study of Endogenous Free L-3-Hydroxybutyrate in H9c2 Cell Line and Rat Tissues
    作者: 徐瑋瑜
    Wei-Yu Hsu
    贡献者: 藥學研究所
    关键词: L-3-羥基丁酸
    H9c2細胞株
    L-3-Hydroxybutyrate
    H9c2 cell line
    日期: 2009
    上传时间: 2009-09-15 15:58:44 (UTC+8)
    摘要: 本論文藉由螢光衍生化試劑搭配逆相高效能層析法，對大鼠各組織中L-3羥基丁酸進行定量。將 4、8、16 和 24 週大的 SD 雄鼠各組織離心後，取上清液與螢光衍生化試劑4 nitro 7 piperazino 2,1,3 benzoxadiazole(NBD PZ)衍生，並利用ODS管柱和串聯的OD-RH管柱進行鏡像異構物的分離。結果發現又以八週大心臟中所定量的L-3HB含量較高(2.10 ± 1.10μM)，而心臟除了L-3HB含量最高之外，所佔的比例也相當高(L%=28.56%)，僅次於比例最高的腎上腺(43.4%)。另外，以心肌H9c2細胞株外加0-10 mM不等的(D+L)-3HB、L 3HB、D 3HB 並培養 0, 2, 10 個小時後，測定在不同時間點細胞內L-3HB的含量。根據實驗結果，外加2 mM D-3HB培養十小時後，心肌細胞株中L-3HB 會大量增加，代表 D-3HB可能是L-3HB的前驅物。

    The concentrations of L-3-hydroxybutyrate (L-3HB) in rat various tissues were determined by column-switching HPLC with pre-column fluorescence derivatization. The tissues of 4, 8, 16, 24-week-old male Sprague–Dawley rats were homogenized and the supernatants were derivatized with a fluorescent reagent, 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ), then separated on an ODS column followed by two-OD-RH-connected chiral columns for enantioseparation. The results showed that the contents of L-3HB were highest in the heart (2.10 ± 1.10 μM, 8 weeks of age). In the adrenal gland, the percentage of L-3HB was found to compose as high as 43.4% of total 3HB, followed by 28.6% in heart homogenate. We further investigated L-3HB levels in heart-derived H9c2 cell line in the presence or absence of 0-10 μM (D+L)-3HB, L-3HB, D-3HB in the medium. After incubation with 2 mM D-3HB for 10 hrs, the concentration of L-3HB in the cells was increased significantly. The result suggests that D-3HB is a precursor of L-3HB.
    数据类型: thesis
    显示于类别:[藥學系] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    摘要.doc27KbMicrosoft Word221检视/开启
    摘要.pdf107KbAdobe PDF201检视/开启
    摘要.ppt105KbMicrosoft Powerpoint235检视/开启
    摘要.ps417KbPostscript101检视/开启


    在TMUIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    著作權聲明 Copyright Notice

    • 本平台之數位內容為臺北醫學大學所收錄之機構典藏，包含體系內各式學術著作及學術產出。秉持開放取用的精神，提供使用者進行資料檢索、下載與取用，惟仍請適度、合理地於合法範圍內使用本平台之內容，以尊重著作權人之權益。商業上之利用，請先取得著作權人之授權。

      The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.

    • 瀏覽或使用本平台，視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例，並不得為任何不法目的使用TMUIR。

      By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.

    • 本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者，煩請權利人通知本平台維護人員([email protected])，將立即採取移除該數位著作等補救措施。

      TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

    Back to Top
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈