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    題名: 結締組織生長因子刺激人類單核球提升CXCL8之分泌
    Investigation of components of vaccinia viral protein complex involved in virus entry
    作者: 林昱良
    Yu-Liang Lin
    貢獻者: 醫學科學研究所
    關鍵詞: 結締組織生長因子
    人類單核球提升
    介白質-8
    CXCL8/IL-8
    Human Monocytes
    Connective Tissue Growth Factor
    CTGF/CCN2
    日期: 2009
    上傳時間: 2009-09-15 11:04:43 (UTC+8)
    摘要: 本篇論文主要的研究重點在於探討Connective Tissue Growth Factor (CCN2/CTGF)和Interleukin-8 (CXCL8/IL-8)兩者之間的關係，也就是希望可以定義出CTGF經由哪一條訊息傳遞路徑來引發IL-8 proinflammatory chemokine的產生進而造成先天免疫反應或是發炎反應。我們利用了酵素結合免疫吸附法(Enzyme-linked immunosobent assay；ELISA) 證實CTGF刺激會引發某些特定細胞IL-8分泌的提升。而此刺激可能作用於mRNA level，而造成IL-8分泌的提升。利用Real-Time qPCR，發現IL-8 mRNA表現量在細胞受到CTGF刺激後表現量會有所上升。利用三種MAPK inhibitor，包括SP600125 (JNK抑制劑)、SB203580 (p38抑制劑)、PD98059 (ERK抑制劑)，發現在SB203580或是SP600125作用下對於CTGF造成的影響，不論是在IL-8 protein level或是IL-8 mRNA level都有一定程度的抑制效果，而PD98059則是效果不顯著，我們也就認為CTGF很可能經由活化p38 或是JNK來引發IL-8的表現提升，抑或是兩者都有參與其中。進一步利用Western blot analysis來實際觀察三種MAPK，JNK、p38、ERK的活化情形，發現phospho-p38的表現在CTGF刺激60分鐘達到最高而phospho-JNK則是在CTGF刺激30分鐘達到最高，而phospho-ERK則是未見其表現，證實了CTGF經由活化JNK及p38 MAPK而刺激人類CD14+單核球細胞分泌IL-8。利用Chromatin Immunoprecipitation證實轉錄因子AP-1及NF-κB會參與CTGF所引發IL-8分泌的提升。因此我們確定CTGF會經由活化p38及JNK MAPK進而使得轉錄因子AP-1及NF-κB結合至IL-8 gene promoter而引發IL-8分泌的提升。

    Connective tissue growth factor (CTGF), a secreted protein that modulates the fibrosis, cell adhesion, cell migration, differentiation, proliferation, angiogenesis behavior of certain cells. However, the effect of CTGF on cytokine secretion in human CD14+ monocytes is mostly unknown. Here we found that CTGF increased the secretion and expression of IL-8 (Interleukin-8) in human CD14+ monocytes. Transcriptional inhibitor (Actinomycin D) inhibited the CTGF-induced secretion of IL-8 of human CD14+ monocytes. JNK MAPK kinase inhibitors (SP600125) and p38 MAPK kinase inhibitors (SB203580) both inhibited the CTGF-induced secretion of IL-8 in human CD14+ monocytes. CTGF stimulation increased the phosphorylation of JNK and p38. In addition, we used Chromatin Immunoprecipitation to observe transcription factors AP-1 and NF-κB both binds to IL-8 gene promoter. Taken together, our results indicated that CTGF enhances the secretion of IL-8 of human CD14+ monocytes through the JNK, p38, AP-1, and NF-κB signal transduction pathway.
    資料類型: thesis
    顯示於類別:[醫學科學研究所] 博碩士論文

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