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| 題名: | Quercetin-3,5,7,3',4'-O-pentamethylether 抑制卵蛋白－引起的氣道過度反應
Quercetin-3,5,7,3',4'-O-pentamethylether inhibits ovalbumin-induced airway hyperresponsiveness |
| 作者: | 蔡淑玫
Shu-Mei Tsai |
| 貢獻者: | 藥理學研究所 |
| 關鍵詞: | 巨噬細胞
小白鼠
白血球
選擇性
抗氣喘
Quercetin-3
5
7
3'&apos
4''-O-pentamethylether+ovalbumin+airway hyperresponsiveness |
| 日期: | 2006 |
| 上傳時間: | 2009-09-11 17:34:05 (UTC+8) |
| 摘要: | Quercetin-3,5,7,3',4'-O-pentamethylether (QPME) 對PDE1-4抑制的IC50值< 10 μM，其PDE4H/PDE4L為11，與目前臨床試驗中最具潛力的抗氣喘藥AWD 12-281相同，本篇實驗將進一步探討QPME是否具有抗氣喘的功效。
在vivo方面，將BALB/c小白鼠腹腔內注射卵蛋白 (ovalbumin, OVA)，使其敏感化，再以卵蛋白氣化噴霧二次激釁 (secondary challenge) 後，利用整體體積描述器 (whole-body plethysmograph) 來分析因 methacholine (MCh, 6.25-50 mg/ml) 引起的氣道過度反應 (airway hyperresponsiveness response,AHR)，結果顯示QPME (10~100 mol/kg, i.p.) 能劑量依存性地減少因MCh (25~50 mg/ml) 噴霧而增加的 Penh值，QPME (100 mol/kg) 亦能有意義地抑制因 MCh (25 mg/ml) 增加的Penh值，QPME (10-100 mol/kg) 也有意義地抑制肺泡灌流液 (BALF) 之總發炎細胞數、巨噬細胞、淋巴球、嗜中性白血球及嗜酸性白血球，並有意義地降低 IL-2、IL-4、IL-5、IFN-γ及TNF- 的釋放，雖然有些例外，即最低劑量不能抑制總發炎細胞數、巨噬細胞、IL-5及IFN-γ的釋放。
在vitro方面，QPME (30~100 M) 能有意義地鬆弛基本張力及抑制 OVA (10~100 g/ml) 引起的敏感化天竺鼠氣管之收縮。
由Lineweaver-Burk分析發現QPME (3~30 M) 對PDE1、PDE2、PDE3及PDE4呈現競爭性的抑制，所得Ki值分別為0.89, 1.07, 0.53及0.52 M，彼此間無意義差，顯示無特殊選擇性，可能由於無選擇性抑制PDE1-4，而增加細胞內cAMP，所以才有抗發炎及抗氣喘的功效。
Quercetin-3,5,7,3',4'-O-pentamethylether (QPME) inhibited activities of PDE1~4, with IC50 values < 10 M. The PDE4H/PDE4L ratio of QPME is about 11, equal to that of AWD 12-281 which is in clinical trial phase II. QPME whether possesses anti-asthmatic effect is the aim of this investigation.
In vivo, female BALB/c mice were sensitized by an intraperitoneal injection of ovalbumin (OVA), then challenged via the airway by ultrasonic nebulization of 1% OVA two periods (secondary challenge). After secondary challenge, the airway hyperresponsiveness (AHR) was measured in unrestrained animals, nebulized with methacholine (MCh, 6.25~50 mg/ml), by barometric plethysmography using a whole-body plethysmograph. In the present results, QPME (10~100 mol/kg, i.p.) dose-dependently attenuated the enhanced pause (Penh) value induced by MCh (25~50 mg/ml). Furthermore, QPME (100 mol/kg, i.p.) also significantly inhibited MCh (25 mg/ml)-induced Penh value. QPME (10-100 mol/kg, i.p.) also significantly inhibited total inflammatory cells, macrophages, neutrophils , lymphocytes, and eosinophils in BALF after determination of Penh values. It also significantly attenuated the release of IL-2, IL-4, IL-5, IFN-γ, and TNF-α, with some exceptions that QPME at the least dose did not suppress releases of total inflammatory cells, macrophages, IL-5, and IFN-γ.
In vitro, QPME (30~100 M) significantly relaxed baseline tension and inhibited cumulative OVA (10~100 μg/ml)-induced contractions in isolated sensitized guinea pig trachealis.
According to the Lineweaver-Burk analysis, QPME (3~30 M) competitively inhibited PDE1, PDE2, PDE3, and PDE4 activities. The Ki values were 0.89, 1.07, 0.53, and 0.52 M which did not differ each other. Owing to QPME did not selectively inhibited PDE1-4, and resulted increase of intracellular cAMP, it may possess anti-inflammatory and anti-asthmatic effects. |
| 資料類型: | thesis |
| 顯示於類別: | [藥學系] 博碩士論文
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