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| 題名: | Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells |
| 作者: | 姚智榮
Ruey-Ho Kao, Gi-Ming Lai, Jyh-Ming Chow, Chien-Huang Liao, Yu-Mei Zheng, Wei-Lun Tsai, Simon Hsia, I-Chun Lai, Hsin-Lun Lee, Shuang-En Chuang, Jacqueline Whang-Peng, Chih-Jung Yao |
| 貢獻者: | 醫學系內科學科 |
| 日期: | 2018-10 |
| 上傳時間: | 2025-04-01 21:07:54 (UTC+8) |
| 摘要: | Abstract
Selenium has been intensively studied for the use of cancer prevention and treatment. However, the clinical effects are still plausible. To enhance its efficacy, a combinational study of selenium yeast (SY) and fish oil (FO) was performed in A549, CL1-0, H1299, HCC827 lung adenocarcinoma (LADC) cells to investigate the enhancement in apoptosis induction and underlying mechanism. By sulforhodamine B staining, Western blot and flow cytometric assays, we found a synergism between SY and FO in growth inhibition and apoptosis induction of LADC cells. In contrast, the fetal lung fibroblast cells (MRC-5) were unsusceptible to this combination effect. FO synergized SY-induced apoptosis of A549 cells, accompanied with synergistic activation of AMP-activated protein kinase (AMPK) and reduction of Cyclooxygenase (COX)-2 and β-catenin. Particularly, combining with FO not only enhanced the SY-elevated proapoptotic endoplasmic reticulum (ER) stress marker CCAAT/enhancer-binding protein homologous protein (CHOP), but also reduced the cytoprotective glucose regulated protein of molecular weight 78 kDa (GRP78). Consequently, the CHOP downstream targets such as phospho-JNK and death receptor 5 were also elevated, along with the cleavage of caspase-8, -3, and the ER stress-related caspase-4. Accordingly, inhibition of AMPK by compound C diminished the synergistic apoptosis induction, and elevated CHOP/GRP78 ratio by SY combined with FO. The AMPK-dependent synergism suggests the combination of SY and FO for chemoprevention and integrative treatment of LADC. |
| 關聯: | Nutrients. 2018 Oct 8; 10(10): 1458 |
| 描述: | 【109-2 升等】臺北醫學大學教師升等專門著作
職別：兼任
送審等級：副教授
著作送審 |
| 資料類型: | article |
| 顯示於類別: | [教師升等送審著作] 109
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