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| 題名: | Ex Vivo Expansion and Drug Sensitivity Profiling of Circulating Tumor Cells from Patients with Small Cell Lung Cancer |
| 作者: | 陳盈汝
Hsin-Lun Lee, Jeng-Fong Chiou, Peng-Yuan Wang, Long-Sheng Lu, Chia-Ning Shen, Han-Lin Hsu, Thierry Burnouf, Lai-Lei Ting, Pai-Chien Chou, Chi-Li Chung, Kai-Ling Lee, Her-Shyong Shiah, Yen-Lin Liu, Yin-Ju Chen |
| 貢獻者: | 生醫材料暨組織工程研究所 |
| 日期: | 2020-11 |
| 上傳時間: | 2025-04-01 19:35:09 (UTC+8) |
| 摘要: | Abstract
Small cell lung cancer (SCLC) represents one of the most aggressive malignancies among cancer types. Not only tumor sample availability is limited, but also the ability for tumor cells to rapidly acquire drug resistance are the rate-limiting bottlenecks for overall survival in current clinical settings. A liquid biopsy capable of capturing and enriching circulating tumor cells (CTCs), together with the possibility of drug screening, is a promising solution. Here, we illustrate the development of a highly efficient ex vivo CTC expansion system based on binary colloidal crystals substrate. Clinical samples were enrolled from 22 patients with SCLC in the study. The CTCs were enriched and expanded from the collected peripheral blood samples. Expanded cells were analyzed for protein expression and observed for drug sensitivity with the use of immunofluorescence and ATP titer evaluation, respectively. Successful CTC spheroid proliferation was established after 4 weeks within 82% of all the collected peripheral blood samples from enrolled patients. Upon immunofluorescence analysis, the enriched cells showed positive markers for EpCAM, TTF-1, synaptophysin and negative for CD45. Additionally, the expanded CTCs demonstrated marked heterogeneity in the expression of E-cadherin and N-cadherin. In a preliminary case series, the drug sensitivity of patient-derived CTC to cisplatin and etoposide was studied to see the correlation with the corresponding therapeutic outcome. In conclusion, our study demonstrates that it is possible to efficiently expand CTCs from SCLC within a clinically relevant time frame; the biomarker information generated from enriched CTCs can assist the selection of effective drugs and improve disease outcome. |
| 關聯: | Cancers (Basel). 2020 Nov 16; 12(11): 3394 |
| 描述: | 【110-1 升等】臺北醫學大學教師升等專門著作
職別：專任
送審等級：副教授
著作送審 |
| 資料類型: | article |
| 顯示於類別: | [教師升等送審著作] 110
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