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| 題名: | Correlation Between Drug Sensitivity Profiles of Circulating Tumor Cell-Derived Organoids and Clinical Treatment Response in Pancreatic Ductal Adenocarcinoma Patients |
| 作者: | 呂隆昇
Yuan-Hung Wu, Yi-Ping Hung, Nai-Chi Chiu, Rheun-Chuan Lee, Chung-Pin Li, Yee Chao, Yi-Ming Shyr, Shin-E Wang, Shih-Chin Chen, Sheng-Hsuan Lin, Yi-Hsuan Chen, Yu-Mei Kang, Shih-Ming Hsu, Sang-Hue Yen, Jeng-You Wu, Kuan-Der Lee, Huey-En Tseng, Jia-Ruey Tsai, Jui-Hsiang Tang, Jeng-Fong Chiou, Thierry Burnouf, Yin-Ju Chen, Peng-Yuan Wang, Long-Sheng Lu |
| 貢獻者: | 生醫材料暨組織工程研究所 |
| 關鍵詞: | Keywords: Antitumour drug screening assays;Circulating tumour cells;Liquid biopsy;Medical oncology;Pancreatic ductal carcinoma;Personalised medicine |
| 日期: | 2022-05 |
| 上傳時間: | 2025-03-31 11:44:58 (UTC+8) |
| 摘要: | Abstract
Introduction: Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive and has poor prognosis. There are few biomarkers to inform treatment decisions, and collecting tumour samples for testing is challenging.
Methods: Circulating tumour cells (CTCs) from patients with PDAC liquid biopsies were expanded ex vivo to form CTC-derived organoid cultures, using a laboratory-developed biomimetic cell culture system. CTC-derived organoids were tested for sensitivity to a PDAC panel of nine drugs, with tests conducted in triplicate, and a weighted cytotoxicity score (CTS) was calculated from the results. Clinical response to treatment in patients was evaluated using Response Evaluation Criteria in Solid Tumours (RECIST) version 1.1 criteria at the time of blood sampling and 3 months later. The correlation between CTS and clinical response was then assessed.
Results: A total of 41 liquid biopsies (87.8% from patients with Stage 4 disease) were collected from 31 patients. The CTC-derived organoid expansion was achieved in 3 weeks, with 87.8% culture efficiency. CTC-derived organoid cultures were positive for EpCAM staining and negative for CD45 staining in the surface marker analysis. All patients had received a median of two lines of treatment prior to enrolment and prospective utility analysis indicated significant correlation of CTS with clinical treatment response. Two representative case studies are also presented to illustrate the relevant clinical contexts.
Conclusions: CTCs were expanded from patients with PDAC liquid biopsies with a high success rate. Drug sensitivity profiles from CTC-derived organoid cultures correlated meaningfully with treatment response. Further studies are warranted to validate the predictive potential for this approach. |
| 關聯: | Eur J Cancer. 2022 May: 166: 208-218 |
| 描述: | 【111-1 升等】臺北醫學大學教師升等專門著作
職別：專任
送審等級：副教授
著作送審 |
| 資料類型: | article |
| 顯示於類別: | [教師升等送審著作] 111
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