Immature Testicular Tissue Engineered from Weaned Mice to Adults for Prepubertal Fertility Preservation—An In Vivo Translational Study

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題名:	Immature Testicular Tissue Engineered from Weaned Mice to Adults for Prepubertal Fertility Preservation—An In Vivo Translational Study
作者:	陳啟煌 How Tseng, Yung-Liang Liu, Buo-Jia Lu, Chi-Huang Chen
貢獻者:	醫學系婦產學科
關鍵詞:	Keywords: bioluminescent imaging (BLI);fertility preservation;immature testis tissue (ITT);poly-l-lactic acid (PLLA);scaffold;spermatogenesis;tissue engineering;transgenic mouse.
日期:	2022-02
上傳時間:	2025-03-28 16:42:41 (UTC+8)
摘要:	Abstract Male pediatric survivors of cancers and bone marrow transplantation often require adjuvant chemoradiation therapy that may be gonadotoxic. The optimal methods to preserve fertility in these prepubertal males are still under investigation. This manuscript presents an in vivo experiment which involved transplantation of immature testicular tissues (ITT) from transgenic donor, to wild-type recipient mice. Donors and recipients were age-mismatched (from 20-week-old donors to 3-week-old recipients, and vice versa) and the transplantation sites involved the abdomen, skin of the head, back muscle, and scrotum. The application of poly-l-lactic acid (PLLA) scaffold was also evaluated in age-matched donors and recipients (both 3-weeks-old). To quantitively evaluate the process of spermatogenesis after ITT transplantation and scaffold application, bioluminescence imaging (BLI) was employed. Our result showed that ITT from 3-week-old mice had the best potential for spermatogenesis, and the optimal transplantation site was in the scrotum. Spermatogenesis was observed in recipient mice up to 51 days after transplantation, and up to the 85th day if scaffold was used. The peak of spermatogenesis occurred between the 42nd and 55th days in the scaffold group. This animal model may serve as a framework for further studies in prepubertal male fertility preservation.
關聯:	Int J Mol Sci. 2022 Feb 12 ; 23(4) : 2042
描述:	【111-2 升等】臺北醫學大學教師升等專門著作職別：專任教師送審等級：教授著作送審
資料類型:	article
顯示於類別:	[教師升等送審著作] 111 [婦產學科] 期刊論文

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