Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ)

Taipei Medical University Institutional Repository > 教師升等著作 > 教師升等送審著作 > 112 > Item 987654321/64812

請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/64812

題名:	Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ)
作者:	吳駿翃 Sani, Saidu;Lawal, Bashir;Ejeje, Jerius N;Aliu, Tawakalitu B;Onikanni, Amos S;Uchewa, Onwe O;Joy, C Ovoh 1;Ekpa, Faith U;Chikezie, D Ozoagu 1;Akuma, Tochukwu S;Onyeji, Success C;Obialor, Amara;Alotaibi, Saqer S;Albogami, Sarah M;Waard, Michel De;Batiha, Gaber El-Saber;Huang, Tse Hung;Wu, Alexander T H
貢獻者:	醫學科技學院轉譯醫學博士學位學程
關鍵詞:	Antioxidant;Diabetes mellitus;Organ histopathology;Oxidative stress;Solanum torvum Swartz.
日期:	2022-10
上傳時間:	2024-12-04 14:04:22 (UTC+8)
摘要:	The current study evaluated the protective role of Solanum torvum Swartz against diabetes-induced oxidative stress and tissue impairment in streptozotocin (STZ)-intoxicated rats. Rats with STZ (40 mg/kg intraperitoneally (i.p.))-induced diabetes were divided into five groups (n = 5) and treated with (i) normal saline; (ii) 150 mg/kg body weight (BW) of the ethanol extract of S. torvum leaf (EESTL); (ii) 300 mg/kg BW EESTL; (iv) 100 mg/kg BW metformin; and (v) 50 m/kg BW metformin + 100 mg/kg BW EESTL orally for 21 days. Our results revealed that the EESTL displayed dose-dependent ferric-reducing antioxidant power (FRAP) activity; scavenged DPPH radicals (IC50) = 13.52 ± 0.45 ?g/mL); and inhibited lipid peroxidation in an in vitro models. In addition; the EESTL demonstrated dose-dependent inhibitory activity against α-amylase (IC50 =138.46 ± 3.97 ?g/mL) and promoted glucose uptake across plasma membranes of yeast cells in a manner comparable to that of metformin. Interestingly; the extract demonstrated in vivo blood glucose normalization effects with concomitant increased activities of antioxidant parameters (superoxide dismutase (SOD); catalase; and reduced glutathione (GSH)) while decreasing malondialdehyde (MDA) levels when compared to untreated rats. Similarly; serum biochemical alterations; and tissues (liver; kidney; and pancreases) histopathological aberrations in untreated rats with STZ-induced diabetes were attenuated by treatment with the EESTL. Biometabolite characterization of the extract identified gallic acid (45.81 ppm); catechin (1.18 ppm); p-coumaric acid (1.43e-1 ppm); DL-proline 5-oxo-methyl ester (9.16 %; retention time (RT): 8.57 min); salicylic acid (3.26% and 7.61 min); and butylated hydroxytoluene (4.75%; RT: 10.18 min) as the major polyphenolic compounds in the plant extract. In conclusion; our study provides preclinical evidence of the antioxidant properties and oxidative stress-preventing role of S. torvum in STZ-dosed diabetic rats. Taken together; the EESTL represents a reserve of bioactive metabolites for managing diabetes and associated complications.
關聯:	Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie; 154; 113605
描述:	【112-2 升等】臺北醫學大學教師升等專門著作職別：專任送審等級：教授著作送審
附註:	代表著作名稱：《Biochemical and tissue physiopathological evaluation of the preclinical efficacy of Solanum torvum Swartz leaves for treating oxidative impairment in rats administered a β-cell-toxicant (STZ)》 Copyright ? 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
資料類型:	article
顯示於類別:	[轉譯醫學博士學位學程] 期刊論文 [教師升等送審著作] 112

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	32	檢視/開啟

在TMUIR中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....