Design and Development of Perfusion System for Studying Surface Shear-Induced Stress for Tissue Engineered Constructs

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Title:	Design and Development of Perfusion System for Studying Surface Shear-Induced Stress for Tissue Engineered Constructs
Authors:	Lim, Joshua M.
Contributors:	奈米醫學工程研究所碩士班 Lin, Chih-Hsin Chen, Yi-Ping
Keywords:	Microfluidics;Shear Stress;Computational Fluid Dynamics;GelMA;Perfusion system
Date:	2024-07-17
Issue Date:	2024-11-06 15:23:02 (UTC+8)
Abstract:	Vasculature-on-a-chip platforms have been widely regarded as an essential tool in addressing the key challenge of forming vascular networks in tissue engineered constructs, as they offer a unique advantage of providing a controlled microenvironments that can be engineered to closely resemble the complexity and dynamic nature of the blood vasculature. In this study, we focused on the design and development of a simple perfusion system to investigate the effects of surface shear-induced stress on tissue-engineered constructs. The developed microfluidic device comprises a central tissue chamber and was fabricated using a combined approach in 3D printing and soft lithography. Computational fluid dynamics simulations were carried out to determine the necessary flow rate that would generate the appropriate shear stress, which should resemble the mechanical microenvironment in vivo. Next, to create the vascularized tissue-engineered construct, the study encapsulated human umbilical vein endothelial cells (HUVECs) within Gelatin Methacrylate 1 hydrogel and the optimal cellular microenvironment was determined by examining the cell viability in various GelMA concentrations. Thereafter, we validated the perfusion system by utilizing the optimal concentration of the HUVEC-laden GelMA hydrogel to form dome-shaped structures, which were then placed at the central tissue chamber of the microfluidic device. The formation of vascular-like networks was then assessed over the course of one week in culture. The current results were not able to demonstrate the effectiveness of shear stress in inducing vascularization. However, we observed stronger colony formation of endothelial cells under dynamic conditions. Nonetheless, we successfully developed a new cell culture perfusion system that is capable of applying physiologically relevant shear stress to any tissue-engineered constructs. Thus, providing a valuable tool for studying the interactions between mechanical stress and engineered tissues.
Description:	碩士指導教授：Lin, Chih-Hsin 共同指導教授：Chen, Yi-Ping 口試委員：Lin, Chih-Hsin 口試委員：Chen, Yi-Ping 口試委員：David J. Lundy 口試委員：Wong, Pei 口試委員：Chen, Zhao Chi
Note:	論文公開日期：9999-12-31
Data Type:	thesis
Appears in Collections:	[Graduate Institute of Nanomedicine and Medical Engineering] Dissertations/Theses

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