衣藻負載乙二醇殼聚醣-聚?咯奈米粒子用作免疫調控及光熱療法之膀胱癌治療

Taipei Medical University Institutional Repository > 醫學工程學院 > 生醫材料暨組織工程研究所 > 博碩士論文 > Item 987654321/64488

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題名:	衣藻負載乙二醇殼聚醣-聚?咯奈米粒子用作免疫調控及光熱療法之膀胱癌治療 Chlamydomonas loaded ethylene glycol chitosan-polypyrrole nanoparticles for immune modulation and photothermal therapy for bladder cancer treatment
作者:	蕭淇紘 HSIAO, CHI-HUNG
貢獻者:	生醫材料暨組織工程研究所碩士班莊爾元潘玟?
關鍵詞:	: 免疫調節、光療作用、腫瘤微環境、衣藻、膀胱癌、乙二醇殼聚醣、?咯、微型載體 Immunomodulation、Phototherapy、Tumor Microenvironment、Chlamydomonas、Bladder cancer、Glycol chitosan、Pyrrole、Microcarrier
日期:	2024-07-09
上傳時間:	2024-10-24 15:01:15 (UTC+8)
摘要:	基於精準光療與免疫調節的合併治療可提供有效的抗腫瘤作用。設計了一種基於光趨化、光合作用和光治療的衣藻負載乙二醇殼聚醣-聚?咯奈米粒子，用以增強免疫結合細胞毒性T細胞和M1/M2巨噬細胞的腫瘤微環境轉換的聯合療法，該療法基於光熱乙二醇殼聚醣聚?咯奈米顆粒（GCS-PPy NPs）負載到具有趨光和光合作用特性的衣藻（CHL）上。基於CHL-GCS-PPy NPs的光療可透過緩解缺氧、調節腫瘤免疫微環境並聯合光熱療效果誘導腫瘤細胞死亡。從體外細胞實驗可以看到膀胱癌細胞通過CHL-GCS-PPy NPs實施光療後，存活率會大幅下降，這種氧化應激的方式不只可以促使癌細胞死亡，還能夠誘導巨噬細胞極化為M1促炎型。從C57BL/6小鼠膀胱癌模型中驗證了這種載體的有效性及安全性。這種基於微藻的系統是開發光療和免疫治療模型的突破，具有活躍的趨化性和有效的微環境調節。它擴展了微生物在生物醫學領域的生物醫學應用，特別是增強腫瘤治療中的光療和免疫治療效果，為臨床腫瘤治療提供了一種有前景的新治療策略。 Combination therapy based on precision phototherapy and immune modulation can provide effective antitumor effects. Designed a Chlamydomonas-loaded ethylene glycol chitosan-polypyrrole nanoparticle based on photochemotaxis, photosynthesis and light therapy to enhance immune binding to cytotoxic T cells and M1/M2 macrophages in the tumor microenvironment Converted combination therapy based on photothermal glycol-chitosan-polypyrrole nanoparticles (GCS-PPy NPs) loaded onto Chlamydomonas algae (CHL) with phototactic and photosynthetic properties. Phototherapy based on CHL-GCS-PPy NPs can induce tumor cell death by relieving hypoxia, regulating the tumor immune microenvironment, and combined with the effect of photothermal therapy. From in vitro cell experiments, it can be seen that the survival rate of bladder cancer cells will decrease significantly after phototherapy with CHL-GCS-PPy NPs. This oxidative stress method can not only promote the death of cancer cells, but also induce the polarization of macrophages. M1 pro-inflammatory type. The effectiveness and safety of this vector were verified in the C57BL/6 mouse bladder cancer model. This microalgae-based system is a breakthrough in developing phototherapy and immunotherapy models with active chemotaxis and effective microenvironmental regulation. It expands the biomedical applications of microorganisms in the field of biomedicine, especially enhances the effects of phototherapy and immunotherapy in tumor treatment, and provides a promising new treatment strategy for clinical tumor treatment.
描述:	碩士指導教授：莊爾元共同指導教授：潘玟? 口試委員：郭聰榮口試委員：劉家宏口試委員：翁佩韋口試委員：莊爾元口試委員：潘玟?
附註:	論文公開日期：2024-07-17
資料類型:	thesis
顯示於類別:	[生醫材料暨組織工程研究所] 博碩士論文

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