| 摘要: | 血栓形成是全球嚴重的健康威脅，死亡率和發病率都很高。目前臨床治療面臨溶栓藥物生物利用率低、血栓形成復發、缺血性缺氧損傷和神經退化等一系列挑戰。本實驗開發了一種具有光/磁能力的生物相容性的萊茵衣藻微馬達（CHL），用來滿足這些需求。這些CHL微馬達配備了趨光性和光合作用的能力，提供了有前途的解決方案。這項創新的研究其核心方面涉及將多醣（乙二醇殼聚醣（GCS）和褐藻醣膠 （F））摻入鐵金屬有機框架（MOF）中，並使其負載尿激?（UK），然後自組裝到多功能平台CHL上，從而形成核殼微結構（CHL@GCS/F-UK-MOF）。在光趨向性的引導下，CHL@GCS/F-UK-MOF被證明可以穿透血栓模型，並增強溶栓效果。同時將CHL@GCS/F-UK-MOF與磁熱療技術相結合，可實現磁熱升溫使金屬磁性導體加熱，從而加速溶栓並快速恢復阻塞的血管。這種方法可減輕血栓誘導的缺血性缺氧疾病和組織損傷。並且CHL@GCS/F-UK-MOF的光合作用和磁熱治療特性，加上它們的保護作用，如減少細胞凋亡、增強行為能力、誘導HSP、極化M2巨噬細胞和緩解缺氧環境，並通過生化、顯微鏡和行為評估得到這一系列作用的證實。這個多功能平台為心血管管理提供了一種全面的方法，並推動了相關技術的發展。
Thrombosis presents a critical health threat globally, with high mortality and incidence rates. Clinical treatment faces challenges such as low thrombolytic agent bioavailability, thrombosis recurrence, ischemic hypoxia damage, and neural degeneration. This study developed biocompatible Chlamydomonas Reinhardtii micromotors (CHL) with photo/magnetic capabilities to address these needs. These CHL micromotors, equipped with phototaxis and photosynthesis abilities, offer promising solutions. A core aspect of this innovation involves incorporating polysaccharides (glycol chitosan (GCS) and fucoidan (F)) into ferric Metal-organic frameworks (MOFs), loaded with urokinase (UK), and subsequently self-assembled onto the multimodal CHL, forming a core-shell microstructure (CHL@GCS/F-UK-MOF). Under light-navigation, CHL@GCS/F-UK-MOF was shown to penetrate thrombi models, enhancing thrombolytic biodistribution. Combining CHL@GCS/F-UK-MOF with the magnetic hyperthermia technique achieves stimuli-responsive multiple-release, accelerating thrombolysis and rapidly restoring blocked blood vessels. Moreover, this approach attenuates thrombi-induced ischemic hypoxia disorder and tissue damage. The photosynthetic and magnetotherapeutic properties of CHL@GCS/F-UK-MOF, along with their protective effects, including reduced apoptosis, enhanced behavioral function, induced HSP, polarized M2 macrophages, and mitigated hypoxia, are confirmed through biochemical, microscopic, and behavioral assessments. This multifunctional biomimetic platform, integrating photo-magnetic techniques, offers a comprehensive approach to cardiovascular management, advancing related technologies. |
