應用飛秒雷射於鈦六鋁四釩合金進行表面改質對骨整合之影響

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題名:	應用飛秒雷射於鈦六鋁四釩合金進行表面改質對骨整合之影響 Effect of Ti6Al4V alloy surface modification on osseointegration using femtosecond laser
作者:	蔡言欣 TSAI, YAN-SIN
貢獻者:	牙體技術學系碩士班沈永康
關鍵詞:	飛秒雷射;植體;表面改質;微結構;骨整合 Femtosecond laser;Implants;Surface modification;Microstructure;Osseointegration
日期:	2024-06-14
上傳時間:	2024-10-07
摘要:	鈦合金因其具有優異的機械性能和生物相容性，現被廣泛應用於牙科和骨科植體；通常對這些植體進行表面改質以提高生物相容性。大部分市售植體多為經過表面噴砂和化學蝕刻，產生隨機的幾何形狀和表面粗糙度，其導致隨機的骨細胞方向，從而形成疤痕組織。基於此，本論文使用飛秒雷射於鈦六鋁四釩平板表面上加工製造規則的微結構 (溝槽、圓洞及方形)，用以提高植體與組織結合的效率，增強骨細胞的附著、增殖和分化。飛秒?射加工技術具有超短脈衝和極高尖峰功率的特性，對材料加工時，熱影響區小、加工尺寸小和加工?密?高。本論文通過改變飛秒雷射的製程參數 (能量密度、掃描速度、掃描次數和頻率) 來製造不同尺寸的微結構，接著利用雷射顯微鏡對雷射樣品進行觀察及測量表面粗糙度；並利用接觸角測量儀測量樣品的潤濕性。MG63細胞在樣品上培養1、4和7天，並進行細胞增殖CCK-8測試和細胞毒性LDH測試，最後使用掃描電子顯微鏡觀察細胞黏附和形態。接觸角量測結果發現具有微結構的Ti6Al4V更具親水性。CCK-8測試顯示，具有微結構的表面對於細胞的增殖具有正面影響，且細胞活性較高，表面有利於成骨細胞反應；LDH測試顯示，Ti6Al4V對MG63細胞無毒性影響。細胞貼附實驗顯示，溝槽結構具有接觸引導作用，能使細胞沿著溝槽方向生長；而細胞在圓洞及方形結構上則是以隨機方向生長。 Titanium alloys are widely used in dental and orthopedic implants due to their excellent mechanical properties and biocompatibility. These implants were subjected surface modified to improve biocompatibility. Most commercially available implants were subjected to surface sandblasting and chemical etching, resulting in random geometries and surface roughness, which may lead to random bone cell orientations that contribute to scar tissue formation. For this reason, femtosecond laser machining was used in this thesis to create regular microstructures (grooves, holes and squares) on the surface of Ti6Al4V flat plates to improve the efficiency of the combination of implants and tissues and enhance the attachment, proliferation and differentiation of bone cells. Femtosecond laser processing technology has the characteristics of ultra-short pulses duration and extremely high peak power, which enables it to process materials with a small heat-affected zone, small processing size, and high processing precision. This thesis manufactured microstructures of different sizes by varying the process parameters of femtosecond laser (fluence, scanning speed, scanning number and frequency), and then used laser microscope to observe the laser samples and measure the surface roughness. The wettability of the samples were measured by contact angle meter. MG63 cells were cultured on the samples for 1, 4 and 7 days, and cell proliferation CCK-8 assay and cytotoxicity LDH assay were performed. Finally, scanning electron microscopy was used to observe cell adhesion and morphology. Contact angle measurement revealed that the Ti6Al4V with microstructure are more hydrophilic. Results of CCK-8 assay demonstrated that the surface with microstructure has a positive influence on cell proliferation, with higher cell viability, and the surface can be conducive to osteoblast reaction; the LDH assay showed that Ti6Al4V has no toxic effect on MG63 cells. Cell attachment experiments showed that the grooves structure has a contact guidance function and can let cells to grow along the direction of the groove. Cells grew in random directions on the hole and square structures.
描述:	碩士指導教授：沈永康口試委員：許世杰口試委員：范芳瑜口試委員：沈永康
附註:	論文公開日期：2029-06-26
資料類型:	thesis
顯示於類別:	[牙體技術學系] 博碩士論文

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