| 摘要: | 攝護腺癌是男性中最普遍的癌症之一，然而其發病途徑仍未完全理解。我們利用轉錄組學和高通量測序分析，研究癌症診斷標靶基因並探索此基因在癌症生物學中的分子機轉。我們通過分析多種基於網站的攝護腺癌數據集（如GEPIA、UALCAN、cBioPortal、SR Plot、hTFtarget、Genome Browser和MetaCore），探討攝護腺癌進展過程中該基因所參與訊號通路活化的相關性預測。我們發現在原發攝護腺腫瘤中高表達的dysbindin domain-containing 1（DBNDD1）與細胞週期、有絲分裂以及轉錄因子結合位點等問題密切相關。DBNDD1基因的表達在攝護腺癌、肝癌和肺癌等不同癌症中有所不同，在不同樣本類型中受到不同癌症階段以及啟動子甲基化程度的影響。在雙相障礙（bipolar disorder）和代謝途徑分析中，我們發現DBNDD1在攝護腺癌中的表現與glycogen synthase kinase-3β(GSK-3β) 訊號通路的調節及其共表達基因密切相關。此外，在攝護腺癌中，DBNDD1基因表達與B細胞、CD4+效應器記憶T細胞和M2巨噬細胞的免疫浸潤呈正相關，但是與CD8+ T細胞、調節性T（Treg）細胞、M1巨噬細胞和NK細胞呈負相關。這些發現說明，DBNDD1不僅可能作為早期攝護腺癌的可行預測標誌物，而且可能對免疫療法也具有預測價值。
Prostate cancer (PCa) ranks as one of the most common forms of cancer in males; its pathogenic pathways remain unknown. Transcriptomics and high-throughput sequencing-based bioinformatics research help discover cancer diagnostic targets and develop better biological network knowledge. Using prostate adenocarcinoma (PRAD) datasets of various web-based applications (GEPIA, UALCAN, cBioPortal, SR Plot, hTFtarget, Genome Browser, and MetaCore), we found that dysbindin domain-containing 1 (DBNDD1) expression was upregulated in primary prostate tumors, exhibiting a significant association with cell cycle issues, mitotic pathways, and transcription factor-binding sites. In addition, factors such as sample type, race, cancer stage, and promoter methylation levels in PRAD influence DBNDD1 expression. Furthermore, DBNDD1 is linked to the regulation of glycogen synthase kinase (GSK)-3β and metabolic pathways in bipolar disorder, as well as immune infiltration in PRAD. DBNDD1 gene expression was positively associated with immune infiltration of B cells, CD4+ effector memory T cells, and M2 macrophages and negatively correlated with CD8+ T cells, regulatory T (Treg) cells, M1 macrophages, and NK cells in PCa. These findings suggest that DBNDD1 may serve as a viable prognostic marker for early-stage PCa and immunotherapies. |
