| 摘要: | 小腸葡萄糖吸收與葡萄糖轉運蛋白 (glucose transporters, GLUT)、甜味味覺接受器 (sweet taste receptor, STR) 和緊密連接 (tight junction) 表現量改變相關，並可以影響體內血糖。非營養性甜味劑 (Non-nutritive sweeteners, NNS) 具有熱量低且高甜度之特性，故常為肥胖、糖尿病患者使用以控制體重和血糖。然而，先前研究較少同時探討與比較人工和天然 NNS 對於小腸葡萄糖吸收的影響，故本研究將透過Caco-2人類結腸腺癌細胞模擬小腸細胞，同時探討並比較人工NNS：蔗糖素 (sucralose, SUC)、醋磺內酯鉀 (acesulfame potassium, ACEK) 以及天然 NNS：甘草素 (glycyrrhizin, GL)、羅漢果萃取物之主要活性成分—羅漢果?V (mogroside V, MGV) 於有無LPS誘導之情況下於葡萄糖吸收及其相關因子的影響。結果顯示介入24小時0.1 mM SUC、0.1 mM ACEK、5 ?M GL與5 ?M MGV於分化14天的Caco-2細胞，皆不影響細胞葡萄糖吸收與相關蛋白質表現量。此外，介入上述NNS會增加sodium-glucose co-transporter 1 (SGLT1)、occludin和claudin1的mRNA表現量於LPS誘導的分化Caco-2細胞，但不影響細胞葡萄糖吸收與培養液中IL-6和IL-8的濃度。綜合以上，人工和天然NNS在有無LPS誘導下於分化Caco-2細胞皆不影響葡萄糖吸收和葡萄糖轉運蛋白與甜味味覺接受器之蛋白質表現量。
Regulation of intestinal glucose absorption is one of the mechanisms to affect blood glucose level, and it is associated with glucose transporter, sweet taste receptors, and tight junction. Non-nutritive sweeteners (NNS) providing few or no calories were introduced as sugar replacement and widely used for individuals with obese and diabetic conditions to control body weight and blood glucose. However, few studies investigated the effect of NNS on intestinal glucose uptake, and compared artificial and natural NNS at the same time. Therefore, the aim was to understand and compare the effects of artificial NNS, including sucralose (SUC), acesulfame potassium (ACEK) and natural NNS, including glycyrrhizin (GL) and mogroside V (MGV), the main bioactive component of monk fruit extract, on glucose transporter, sweet taste receptor, and tight junction in differentiated Caco-2 cell, and the effects on glucose uptake with or without LPS-treated condition. The results showed that 0.1mM SUC, 0.1mM ACEK, 5 ?M GL, and 5 ?M MGV did not affect glucose uptake and the expression of associated protein in differentiated Caco-2 cell. In addition, NNS may increase sodium-glucose co-transporter 1 (SGLT1), occludin, and claudin1 mRNA expression under LPS-treated condition, however did not affect glucose uptake. In summary, NNS did not affect glucose uptake and related protein expression with or without LPS-treated condition. |
