English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 45069/58245 (77%)
造訪人次 : 2341536      線上人數 : 177
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/63129


    題名: 利用光聚合GelMA水凝膠與生長因子結合之生物墨水材料建構一個含有功能性之血管網路系統
    Combining GelMA hydrogel with growth factors as bio-inks for developing functional vascular networks
    作者: 陶宣雅
    TAO, HSUAN-YA
    貢獻者: 奈米醫學工程研究所碩士班
    陳奕平
    林芷歆
    關鍵詞: 明膠甲基丙烯;血管內皮生長因子;成纖維細胞生長因子;血管化
    GelMA;Vascular endothelial growth factor, VEGF;Basic - Fibroblast Growth Factor, bFGF;Vascularization
    日期: 2023-06-27
    上傳時間: 2023-12-07 09:47:44 (UTC+8)
    摘要: 製造具有功能性血管網絡系統的結構一直是建立替代生物組織中的一個重大挑戰,為了克服當前組織工程的困難,我利用三維生物打印策略來開發生物墨水。當中,生物墨水材料由載有生長因子的光交聯甲基丙烯酸明膠(Gelatin Methacrylate, GelMA)複合水凝膠組成,並於血液來源的人臍靜脈內皮細胞(Human umbilical vein endothelial cells, HUVECs)和脂肪來源幹細胞(Adipose-derived stem cells,ADSCs)共同培養之水凝膠模型中形成穩定的脈管系統網絡,研究了從內皮細胞形成血管網絡的新興組織工程方法。本研究提出GelMA與血管生長因子相結合,之後與HUVECs以及ADSCs進行細胞包覆(Cell encapsulation),以模擬體內血管生成和發育過程,並誘導血管組織新生。對於GelMA、GelMA@VEGF、GelMA@FGF這三種不同水凝膠分別以Cell Counting Kit 8 Assay Kit (CCK-8)進行活性測試,在細胞包覆後培養第一周內,微血管網絡形成並保持功能穩定,此後,細胞逐漸形成管腔,在第二週後分化為血管周圍細胞以穩定血管,整個構建體在體外培養期間皆表現出高結構穩定性,驗證細胞在具有生長因子結合之材料中能幫助細胞增長。此外,亦以螢光染色標誌去辨識內含DNA的細胞核及粒線體的位置,由結果證明生長因子水凝膠促進 HUVECs及ADSCs血管生成的潛力,得出此建構體能夠有效支持細胞存活和增殖。
    Fabricating structures with functional vascular network systems has been a major challenge in creating alternative biological tissues. To overcome the current difficulties in tissue engineering, people start looking for the new strategy to find the bio-ink of vascular network formation in tissue engineering. This study proposes to combine photo-crosslinked gelatin methacrylate (GelMA) with vascular growth factors. So as to simulate the process of vasculogenesis and development and try to form a stable vasculature network in a hydrogel model. The whole construct showed high structural stability during 28 days in three hydrogel types, and it was verified that the construct with growth factors can grow better. In addition, fluorescent staining markers proved the potential of vasculogenesis in HUVECs and ADSCs, and concluded that this construct can support cell survival and proliferation effectively.
    描述: 碩士
    指導教授:陳奕平
    共同指導教授:林芷歆
    委員:郭聰榮
    委員:陳奕平
    委員:林芷歆
    委員:楊正昌
    委員:吳思翰
    資料類型: thesis
    顯示於類別:[奈米醫學工程研究所] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML90檢視/開啟


    在TMUIR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    著作權聲明 Copyright Notice
    • 本平台之數位內容為臺北醫學大學所收錄之機構典藏,包含體系內各式學術著作及學術產出。秉持開放取用的精神,提供使用者進行資料檢索、下載與取用,惟仍請適度、合理地於合法範圍內使用本平台之內容,以尊重著作權人之權益。商業上之利用,請先取得著作權人之授權。

      The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.

    • 瀏覽或使用本平台,視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例,並不得為任何不法目的使用TMUIR。

      By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.

    • 本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者,煩請權利人通知本平台維護人員([email protected]),將立即採取移除該數位著作等補救措施。

      TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

    Back to Top
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋