| 摘要: | 目前對於荔枝的研究大多都集中於果肉、果殼及籽，針對樹葉的研究較少，樹枝的部分目前尚未有文獻探討其成分及藥理作用。荔枝在採收的過程同時也會增加農業廢棄物的產生，因此藉由再利用荔枝的樹枝及樹葉，探討兩種荔枝副產品於雄性禿(AGA)中的促生髮作用。於本次的研究中測定了其化學成分及活性成分，並使用人類毛囊乳禿細胞(HFDPC)以及動物實驗，給予二氫睪固酮(DHT)誘導為AGA模型，探討荔枝樹枝(LCT)、荔枝葉(LCL)是否有抑制DHT的作用以達到促進毛髮生長的作用。於本次的研究結果顯示，荔枝樹枝丙酮萃(LCTA)具有較佳的抗氧化能力，但於細胞實驗中，反而是荔枝樹枝水萃(LCTW)和荔枝樹枝酒萃(LCTE)對於毛囊細胞具有恢復DHT造成的損傷。另外在基因表達中也有增加WNT10b、降低雄激素受體(AR)、DKK1的表現。於動物實驗中也可發現，經由DHT造成的毛囊萎縮，同時給予LCTW和LCTE後便可恢復毛囊的生長，於基因表現中，LCTW隨著劑量的增加而促進WNT10b、β-catenin、Lef1、VEGF的表現，也降低了DKK1、IL-6及AR的表現。LCTE則是在低劑量時表達效果最佳。於Western Blot結果中，LCTW對於AR有較好的抑制作用，LCTE則是隨著劑量的上升而促進WNT/β-catenin這條路徑。在5α-reductase 活性試驗中LCTW及LCTE對於5α-reductase並無抑制作用。最後使用HPLC檢測LCTW和LCTE，發現成分大多相似，但LCTE的含量較多。因此綜合本次的研究結果，LCTW和LCTE均可對DHT造成的傷害進行保護作用，未來可著重研究LCT於雄性禿的治療，並同時減少農業廢棄物的積累。
Currently, most of the research on litchi focuses on its pulp, shell, and seed, while studies on its leaves are relatively scarce. There is a lack of literature investigating the composition and pharmacological effects of litchi twigs. During the harvesting process of litchi, it also leads to an increase in agricultural waste generation. Therefore, by utilizing the litchi twigs and leaves as byproducts, this study aims to explore their potential for promoting hair growth in androgenetic alopecia (AGA).In this study, the chemical composition and active constituents of litchi twigs (LCT) and litchi leaves (LCL) were determined. Human Hair Follicle Dermal Papilla Cells (HFDPC) and animal experiments were conducted to investigate the potential inhibitory effects of LCT and LCL on dihydrotestosterone (DHT)-induced AGA, with the aim of promoting hair growth. The results of this study showed that litchi twigs acetone extract (LCTA) exhibited superior antioxidant activity. However, in cell experiments, it was observed that litchi twigs water extract (LCTW) and litchi twigs ethanol extract (LCTE) had the ability to restore the damage caused by DHT on cells. Moreover, there were also increased expressions of WNT10b and decreased expressions of androgen receptor (AR) and DKK1 of gene expression. In animal experiments, it was observed that DHT-induced HFDPC atrophy could be restored by administration of LCTW and LCTE. In gene expression, LCTW demonstrated a dose-dependent increase in the expression of WNT10b, β-catenin, Lef1, and VEGF, while decreasing the expressions of DKK1, IL-6, and AR. LCTE demonstrated its optimal expression effects at low doses. Western blot analysis revealed that LCTW exhibited stronger inhibitory effects on AR, while LCTE showed a dose-dependent promotion of the WNT/β-catenin pathway. In the 5α-reductase activity assay, both LCTW and LCTE showed no inhibitory effects on 5α-reductase. Based on the HPLC analysis, found that the compositions of LCTW and LCTE were mostly similar, but LCTE had a higher content.In this study, shows that both LCTW and LCTE demonstrated protective effects against DHT-induced damage. In the future, it can focus on researching the potential of LCT in the treatment of AGA while also addressing the accumulation of agricultural waste. |
