Taipei Medical University Institutional Repository:Item 987654321/63097
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    題名: 開發微脂體製備與粒線體包埋的微流體裝置並用於細胞治療
    Development of microfluidic devices for liposome preparation and mitochondria encapsulation for cell therapy.
    作者: 許宴菁
    HSU, YEN-CHIN
    貢獻者: 生醫材料暨組織工程研究所碩士班
    劉家宏
    范育睿
    關鍵詞: 微流體;脂質體;線粒體封裝;細胞療法;藥物傳遞
    Microfluidics;Liposomes;Mitochondria Encapsulation;Cell Therapy;Drug Delivery
    日期: 2023-05-23
    上傳時間: 2023-12-07 09:39:33 (UTC+8)
    摘要: 細胞療法是一種醫療干預方法，涉及將細胞傳遞給患者，用於修復或替代受損的組織或器官。然而，這種治療方法的功效在很大程度上取決於移植細胞的存活能力、操作功能和治療潛力。線粒體，俗稱為細胞的“能量工廠”，在保持細胞活力和功能方面起著關鍵作用。增強移植細胞中的線粒體功能可能大幅增強細胞為中心的治療效益。
    實驗主要目標是從細胞中提取線粒體，並將這些器官封裝到脂質體中，以確保線粒體的長期存活，從而實現與細胞療法相關的治療效果。
    我們設計了一個微流控裝置，其中包含多個操作單元。該裝置旨在高效制備脂質體並封裝線粒體。該微流控平台利用精確的流體控制和微尺度特徵，實現均勻的脂質體尺寸和組成。此外，該微流控平台有助於在脂質體內封裝線粒體，保持其結構完整性和功能活性。
    實驗證明使用微流控裝置製備的脂質體的特性分析顯示，其具有均勻的尺寸分布和高封裝效率的線粒體。被封裝的線粒體保持其生物能量特性和操作功能，證實了它們在增強細胞的存活能力和操作能力方面的潛力，從而確認了它們在治療應用中的潛在作用。
    我們開發的微流控裝置相比傳統的脂質體制備和線粒體封裝方法具有幾個優勢。精確控制流體動力學、混合物和脂質雙層的形成，實現了具有特定特性的脂質體的可重現和可擴展生產。重要的是，將線粒體封裝在脂質體內為線粒體提供了在移植過程中的保護環境，從而優化了它們的傳遞和對受體細胞的影響。
    本文介紹的微流控裝置提出了一種新穎有效的策略，用於準備脂質體並封裝線粒線體，用於細胞療法應用。對脂質體特性和線粒體封裝的精確控制為增強細胞為中心的治療結果提供了潛在的進展。需要進一步研究以探索這些脂質體在相關病理模型中促進細胞存活、功能和組織再生的功效。
    Cell therapy, a medical intervention involving the delivery of cells to a patient, is employed to restore or supplant damaged tissues or organs. Nevertheless, the efficacy of this therapeutic approach hinges significantly on the viability, operational functionality, and therapeutic potential of the transplanted cells. Mitochondria, colloquially known as the 'powerhouses' of cells, are instrumental in preserving cellular vitality and function. Augmenting the mitochondrial functionality in transplanted cells could substantially amplify the therapeutic benefits of cell-centric treatments. The primary aim is to extract mitochondria from cells and encapsulate these organelles into liposomes, verifying the longevity of the mitochondria to achieve the therapeutic effect associated with cell therapy.
    We have engineered a microfluidic apparatus that incorporates multiple operational units. This device has been devised for the efficient preparation of liposomes and the encapsulation of mitochondria. The said apparatus capitalizes on meticulous fluidic control and micro-scale features to attain uniform liposome dimensions and composition. In addition, the microfluidic platform facilitates the encapsulation of mitochondria within liposomes, preserving their structural integrity and functional activity.
    The characterization of liposomes, manufactured utilizing the microfluidic device, revealed a uniform size distribution and a high encapsulation efficacy of mitochondria. The sequestered mitochondria conserved their bioenergetic properties and operational functionality, confirming their potential to bolster cell viability and operationality in therapeutic applications.
    The microfluidic apparatus we've developed offers several advantages compared to traditional methodologies employed for liposome preparation and mitochondrial encapsulation. Precise control over fluid dynamics, mixtures, and the formation of lipid bilayers enables the reproducible and scalable production of liposomes with specified characteristics. Importantly, the incorporation of mitochondrial encapsulation within liposomes offers a protective milieu for the mitochondria during transplantation, thereby optimizing their delivery and subsequent influence on recipient cells.
    The microfluidic apparatus presented herein proposes a novel and efficacious strategy for preparing liposomes and encapsulating mitochondria intended for cell therapy applications. The precise control over liposome characteristics and mitochondrial encapsulation proffers potential advancements in augmenting the therapeutic results of cell-centric therapies. Further research is needed to investigate the efficacy of these liposomes in promoting cell survival, functionality, and tissue regeneration in relevant pathological models.
    描述: 碩士
    指導教授:劉家宏
    共同指導教授:范育睿
    委員:劉家宏
    委員:郭聰榮
    委員:曾靖孋
    委員:范育睿
    委員:莊爾元
    資料類型: thesis
    顯示於類別:[生醫材料暨組織工程研究所] 博碩士論文

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