| 摘要: | Endophytic fungi have a potential source to lead new bioactive compounds because of the diversity of structure and biological activity. There has been no investigation of the structure and biological activity of compounds fermented produced of fungi associated with Melaleuca leucadendra Linn and the marine alga Mastophora rosea. This study, investigation of the structure and biological activity compounds of Nigrospora aurantiaca #TMU062 and Trichoderma brevicompactum NTU439 which is exhibited by endophytic fungi from M. leucadendra and marine alga M.a rosea. A new compound (1) along reported compounds (11 – 15) isolated from #TMU062. Moreover, four new compounds (2 – 5) and five reported compounds (6 – 10) isolated from NTU439. The structures of all compounds were determined by 1D/2D NMR (nuclear magnetic resonance), MS (mass spectrometry), and IR (infrared spectroscopy). The cytotoxic activity against HCT-116, PC-3, and SK-Hep-1 cancer cells by the SRB assay, anti-neuroinflammatory assay by inhibitory of nitric oxide (NO) production in LPS-activated microgial BV-2 cells, and gelatinolytic activities of matrix metalloproteinase-9 (MMP-9) in human THP-1 monocytic cell S1 protein-simulated were evaluated of compounds. Compound 14 showed an anti-neuroinflammatory activity (IC50: 7.2 ± 1.4 ?M; survival rate: 90.8 ± 6.7%). Besides, new compounds 2, 3, 5 and compounds 6, 8, 9, 11, and 12 showed potent an anti-neuroinflammatory. Compounds 6 and 9 showed markedly inhibited gelatinolysis and potent cytotoxic activity on all cancer cell line.
Keywords: Nigrospora aurantiaca, Trichoderma brevicompactum, Melaleuca leucadendra, marine alga Mastophora rosea, endophytes fungi, nitric oxide production, trichothecenes, gelatinolytic activities, MMP-9, cytotoxic |
