多孔性骨填補材的特性及生物相容性研究

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題名:	多孔性骨填補材的特性及生物相容性研究 Characterization and biocompatibility of coral-based bone graft
作者:	羅啓中 LO, CHI-CHUNG
貢獻者:	牙體技術學系碩士班沈永康
關鍵詞:	珊瑚繁殖;羟基磷灰石;β-磷酸三钙;珊瑚衍生的双相骨移植物 Propagated coral;Hydroxyapatite;β-tricalcium phosphate;Coralline-derived biphasic bone graft
日期:	2022-01-19
上傳時間:	2022-08-01 11:52:41 (UTC+8)
摘要:	本研究混合人工養殖的石珊瑚和無水磷酸二鈣 (DCPA, CaHPO4)，加熱1100℃，探討從 1 小時到 7 天的熱處理持續時間的影響。並藉由X 射線衍射 (XRD)、傅里葉變換紅外光譜 (FTIR)、拉曼光譜和掃描電子顯微鏡 (SEM) 分析所製備的珊瑚和珊瑚衍生的雙相 CaP。使用小鼠成纖維細胞通過體外細胞毒性評估來評估雙相 CaP 的生物相容性。雙側股骨缺損兔模型評估了珊瑚衍生的雙相CaP骨移植物對早期局部組織反應和生物降解率的影響。基於XRD、FTIR和拉曼光譜證實β-TCP和HAp在1100熱處理1小時時共存，且隨著熱處理時間的增加，HAp/β-TCP的比值增加。包含 61% HAp 和 39% β-TCP (HT-3) 的珊瑚衍生的雙相 CaP 沒有表現出證明其生物相容性的細胞毒性。此外，在 HT-3 和自體骨之間沒有觀察到局部組織反應的顯著差異。 HT-3的殘留量為72.85±9.87。在 1100°C 下熱處理 3 天，合成了 HAp/β-TCP 比率約為 6/4 的珊瑚衍生的雙相 CaP，由於良好的生物相容性並且對組織反應沒有不利影響，因此被認為是骨移植的理想候選者。 Biphasic calcium phosphate ceramics (CaPs) comprising β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAp) were synthesized by the mixing of the propagated Scleractinian coral and dicalcium phosphate anhydrous (DCPA, CaHPO4) followed by the heat treatment at a temperature of 1100 °C. The effects of heat-treated duration from 1 hour to 7 days were examined. The as-prepared coral and coralline-derived biphasic CaPs were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and scanning electron microscopy (SEM). The biocompatibility of the biphasic CaPs was evaluated by in vitro cytotoxicity assessment using mouse fibroblast cells. The bilateral femoral defect rabbit model assessed the effect of the coralline-derived biphasic CaP bone graft on the early local tissue reaction and biodegradation rate. Based on XRD, FTIR, and Raman spectra confirmed the co-existence of β-TCP and HAp at 1100 for 1-hour heat treatment, and with the increase of heat-treated time, the ratio of HAp/β-TCP increased. The coralline-derived biphasic CaP comprising 61% HAp and 39% β-TCP (HT-3) did not exhibit cytotoxicity evidencing its biocompatibility. Furthermore, no significant differences in local tissue reaction were observed between the HT-3 and autogenous bone. The residue of HT-3 was 72.85±9.87. The coralline-derived biphasic CaP with HAp/β-TCP ratio of around 6/4 was synthesized by the mixing of the propagated Scleractinian coral and dicalcium phosphate anhydrous (DCPA, CaHPO4) followed by the heat treatment at 1100 °C for 3 days, which was suggested as an ideal candidate for bone graft due to the well biocompatibility and no adverse effect in the tissue response.
描述:	碩士指導教授:沈永康委員:沈永康委員:范芳瑜委員:林煒竣
資料類型:	thesis
顯示於類別:	[牙體技術學系] 博碩士論文

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