The Effects of Ferric Citrate Supplementation on Omega 3 Polyunsaturated Fatty Acids Biosynthesis in High Fat Diet Induce Obese Rats

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題名:	The Effects of Ferric Citrate Supplementation on Omega 3 Polyunsaturated Fatty Acids Biosynthesis in High Fat Diet Induce Obese Rats
作者:	FARADINA, AMELIA
貢獻者:	保健營養學系碩士班張榮素
關鍵詞:	Omega 3 polyunsaturated fatty acids;Iron supplementation;Obesity;Oxidative stress
日期:	2021-06-24
上傳時間:	2022-01-13 14:01:18 (UTC+8)
摘要:	Background: Obesity is associated with iron deficiency. Currently, obese patients with functional iron deficiency received iron supplementation as the standard treatment method, but the risk of tissue iron overload may further increase due to iron supplementation. Aim: The objective of this study was to elucidate the dose-related effect of ferric citrate supplementation on omega 3 (n-3) Polyunsaturated Fatty Acids (PUFAs) biosynthesis in high fat diet (HFD)-induced obese rats. Methods: Sprague-Dawley (SD) rats were fed with either normal diet or HFD 50% fat without or with ferric citrate supplementation (0.25, 1, or 2 g ferric iron/kg diet) for 12 weeks. Erythrocyte membrane fatty acid (FA) profile was performed by gas chromatograph after lipid extraction and fractionation. Expression of key converting enzymes involved in n-3 PUFAs biosynthesis (Δ-6 desaturase, Δ-5 desaturase, and elongase 2) was detected by western blotting. Results: At week 12, obese rats received 2 g ferric iron/kg diet had decreased levels of eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), docosahexaenoic acid (DHA) and total n-3 PUFAs compared with rats fed with standard diet or HFD alone. Pathological examination of liver showed that iron supplementation induced liver steatosis, inflammation and mild liver iron overload. Ferric citrate supplementation had dose-dependent effect on erythrocyte degradation biomarkers including LVV-hemorphin-7, lipid peroxidation marker malondialdehyde (MDA) and insulin. Furthermore, ≥1g ferric iron decreased n-3 PUFAs converting enzymes Δ6 and Δ5 desaturase by western blot analysis. Spearman correlation analysis showed inverse correlation between total liver iron, serum LVV-hemorphin-7, MDA with n-3 PUFAs and it’s converting enzymes Δ5 and Δ6 desaturase. Conclusion: High dose ferric iron supplementation decreased n-3 PUFAs levels in HFD induced obese rats, possibly acting through iron and fat mediated oxidative stress to down regulate Δ5 and Δ6 desaturase.
描述:	碩士指導教授：張榮素委員：黃士懿委員：劉凱莉
資料類型:	thesis
顯示於類別:	[保健營養學系暨研究所] 博碩士論文

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