| 摘要: | 幾個世紀以來，細菌感染一直是嚴重的健康問題，過去幾十年抗生素的使用使感染更難治療，原因是抗生素的濫用，使細菌增強了對抗生素的抵抗力，從而導致抗生素治療失敗。考慮到上述情況，開發新型抗菌策略具有重要意義。
為了對抗細菌引發的感染，我們尋找毋需依賴抗生素也能對抗細菌的材料，因此我們製造了一種具光熱抗菌活性的二氧化矽奈米複合粒子來解決目前遇到的抗菌難題。我們通過微乳液系統來合成中空二氧化矽奈米粒子(HSN)，並將奈米鑽石(ND)和碳量子點(CQD) 這兩種碳奈米材料裝載至其中，因為已有研究指出ND與CQD皆具有高生物相容性以及結構穩定，可以將兩者作為光熱劑使用運用在光熱治療上(PTT)。在本研究中我們將癸烷作為油相，氟化鈉做為水相，搭配CO-520 (界面活性劑) 並加入 己醇 (助界面活性劑)合成得到的HSN，經過DLS和TEM的鑑定結果為粒徑50nm的奈米空心球，我們利用ND和CQD的螢光特性來鑑定ND和CQD是否有被裝載進HSN中，其結果顯示我們成功將不同濃度的ND和CQD裝載進HSN之中，並成功做出兩種不同的二氧化矽奈米複合材料，且製作出的二氧化矽奈米複合材料並不會產生細胞毒性。在未來的研究中，我們會將裝載ND的二氧化矽奈米複合材料 (ND@HSN)與裝載CQD的二氧化矽奈米複合材料(CQD@HSN)，對大腸桿菌與金黃色葡萄球菌進行光熱治療，並觀察其殺菌效果。目前研究指出ND和CQD被特定的光波長激發後可以釋放出ROS，其可以破壞細菌的生物膜導致細胞死亡，因此我們也會進行光動力治療(PDT)來觀察滅菌的效果。
Bacterial infections have been a serious health problem for centuries. In the past few decades, the use of antibiotics has made infections more difficult to treat. The reason is that the abuse of antibiotics makes bacteria strengthen their resistance to antibiotics, which leads to the failure of antibiotic treatment. Considering the above situation, the development of new antibacterial strategies is of great significance. Considering the above situation, the development of new antibacterial strategies is of great significance.
In order to fight infections caused by bacteria, we are looking for materials that can fight bacteria without relying on antibiotics. Therefore, we have manufactured a kind of photothermal antibacterial activity of silica nanocomposite particles to solve the current antibacterial problems encountered. We use a microemulsion system to synthesize hollow silicon dioxide nanoparticles (HSN), and at the same time load two carbon nanomaterials, nanodiamonds (ND) and carbon quantum dots (CQD), because studies have pointed out that ND and CQD can be used as a photosensitizer and used in photothermal therapy (PTT). In this study, we determined that HSN is a hollow nanosphere with a particle size of 50nm. We used the fluorescence characteristics of ND and CQD to identify whether ND and CQD were loaded into HSN. The results showed that we successfully made two different Nanocomposites can also load different concentrations of ND and CQD into the HSN, and the nanocomposites produced do not produce cytotoxicity. In subsequent research, we will use ND-loaded silica nanocomposite material (ND@HSN) and CQD-loaded silica nanocomposite material (CQD@HSN) to sterilize Escherichia coli and Staphylococcus aureus experiment. |
