奈米金島狀薄膜基板於細菌檢測及生長抑制之應用

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題名:	奈米金島狀薄膜基板於細菌檢測及生長抑制之應用 Application of Gold Nano-island Film as a Substrate for Bacterial Detection and Inhibition
作者:	譚詩樺 TAN, SHIH-HUA
貢獻者:	奈米醫學工程研究所碩士班郭聰榮陳志華
關鍵詞:	奈米金島狀薄膜;侷域性表面電漿共振;表面增強拉曼散射;細菌檢測;抑菌作用 Gold nano-island films;Localized surface plasmon resonance、Surface-enhanced Raman scattering;Bacterial detection;Antibacterial effect
日期:	2021-06-18
上傳時間:	2022-01-04 20:58:04 (UTC+8)
摘要:	細菌感染一直是很棘手的公共衛生議題，且會導致疾病的產生，例如：敗血症、組織感染、腎功能衰竭等，嚴重的話甚至會致命。細菌檢測對於評估感染風險以及防止細菌傳播十分重要，因此需要一種具有高靈敏度及高準確度的細菌檢測技術。奈米金屬材料之侷域性表面電漿共振(localized surface plasmon resonance, LSPR)可以增強拉曼訊號，以提高檢測靈敏度與獲得更好的影像。在本研究中，我們利用奈米金島狀薄膜(gold nano-island film, AuNIFs)作為細菌檢測的平台，同時具有抑制細菌生長的功能。 AuNIFs的製備方法是在溶液相中先將金的晶種沉積於玻璃基板表面上，再將金離子還原至晶種上，使晶種持續長大形成AuNIFs，而其表面結構會使侷域性電場增強，可應用於表面增強拉曼散射(surface-enhanced Raman scattering, SERS)，藉由偵測AuNIFs和細菌間的交互作用力來檢測細菌。研究結果顯示從拉曼光譜圖中可以觀察到E. coli的SERS特徵峰。此外，AuNIFs在白光(300 mW/cm2)的照射下具有良好的光熱轉換效率，可利用其光熱效應所產生之高溫來抑制細菌生長。本研究證明AuNIFs易於製備，可以成為一種快速且具備高靈敏度的細菌檢測平台，同時作為光熱轉換媒介，表現出抑菌效果。 Bacterial infections have always been a serious public health problem. Many pathogenic bacteria can lead to life-threatening diseases, such as sepsis, tissue infections, kidney failure, or even death. The detection of bacteria is important for evaluating the infection risks as well as preventing further bacteria transmission. Therefore, an alternative technique with high sensitivity and specificity is needed. Localized surface plasmon resonance (LSPR) of nano-metal materials has been utilized to enhance Raman signal intensity for better detection and imaging quality. In this study, AuNIFs were developed as a platform for bacterial detection as well as inhibition of bacteria growth. AuNIFs were fabricated through a solution-phase approach by depositing Au seed crystals on the glass substrate and then reduced Au3+ ions onto the Au seed layer to form gold nano-island film. The local electric field enhancement from the gold nano-island structure results in surface-enhanced Raman scattering (SERS). Bacterial detection depends on sensing the direct interaction between AuNIFs and bacteria, and the Raman spectrum showed several typical SERS peaks of E. coli. Furthermore, AuNIFs showed good photothermal performances under white light irradiation (300 mW/cm2), leading to the inhibition of bacteria growth by hyperthermia. Overall, we demonstrated that AuNIFs are easy to prepare, and can be a novel and rapid platform for bacterial detection with high sensitivity as well as a photothermal transducer showing antibacterial effect in vitro.
描述:	碩士指導教授：郭聰榮指導教授：陳志華委員：楊正昌委員：鄭財木委員：王迪彥
資料類型:	thesis
顯示於類別:	[奈米醫學工程研究所] 博碩士論文

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