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    請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/61133


    題名: 開發抗聚乙二醇報告基因表現之細胞與轉殖基因鼠以追蹤細胞命運與移植存活之前期研究
    A Preliminary Study of Generating Anti-Polyethylene Glycol(PEG) Reporter Expressing Cells and Transgenic Mice to Track Cell Fates and Transplant Survival
    作者: 王昌弘
    Wang, Chang-Hung
    貢獻者: 生藥學研究所
    莊國祥
    關鍵詞: 幹細胞;細胞治療;報告基因;器官移植
    Stem Cell;Cell Therapy;Cell Tracking;Reporter Gene;Transplantation
    日期: 2016
    上傳時間: 2021-11-22 16:20:17 (UTC+8)
    摘要: 幹細胞目前在再生醫學與移植醫學已成為解決目前無法治療之疾病的重要議題，但目前幹細胞治療策略開發，大多是以人類幹細胞，施打到正常老鼠或免疫缺陷鼠進行研究，此會造成細胞特性研究與免疫機制無法正確評估，且目前在再生與移植醫學面臨無法長期的追蹤細胞、組織與器官之分布與存活的問題。而先前實驗室開發鼠源性抗聚乙二醇抗體，並開發成鼠源性抗聚乙二醇報告基因(Anti-PEG Reporter)，我們希望利用Anti-PEG Reporter具有高專一性、低免疫性與適用於多種造影系統(如光學造影系統、正子造影系統與核磁共振造影系統)，長期追蹤細胞、組織與器官之分布與存活。第一部分研究為幹細胞研究，我們利用細胞自我更新能力(Cell Self-Renewal)、生物標記(Biomarkers)與細胞分化能力(Cell Differentiations)，已鑑定鼠源性間質幹細胞，且初步發現具有治療四氯化碳誘導急性肝損傷老鼠的能力，並確認可以表現 Anti-PEG Reporter；第二部分為了長期研究組織器官之移植成效與存活，我們委託國家實驗動物中心蔣思澈博士，建構全身表現Anti-PEG Reporter轉殖基因小鼠，我們利用Genotyping、PEG修飾蛋白質藥物與PEG修飾螢光探針之Anti-PEG Reporter功能性實驗與Anti-PEG Reporter蛋白質分析實驗，證實Anti-PEG Reporter確實表現成功與功能性正常。本前期研究之目的為應用Anti-PEG Reporter可長期追蹤、高專一性與適用多種造影系統的特性，進行幹細胞之再生醫學研究與基因轉殖工具鼠之移植醫學研究，長期觀察細胞、組織與器官之分布與存活。
    Stem cell therapy is considered a great regenerative medicine and transplantation material in recent years. However, developing stem cell therapy strategies is an issue. Many research uses normal or immunodeficiency mouse model in human stem cell studies. However, human cell properties and mechanisms are different from the mouse. Therefore, in long-term, the regenerative and transplantation study is hard to observe. To solve this problem, we have developed mouse anti-polyethylene glycol(PEG) antibody and evolved the antibody into Anti-PEG Reporter. Anti-PEG Reporter is a highly specific, low immunogenic and suitable for multiple imaging system (eg. Optical Imaging System, Positron Emission Tomography System (PET), and Magnetic Resonance Imaging (MRI)). Anti-PEG reporter offers long-term tracking that can provide the location and survival of the cells, tissues and organs. First part of the research is stem cell regenerative medicine research. Mesenchymal stem cell (MSC) is confirmed by cell self-renewal, biomarkers and differentiations, and is proven that it is able to cure CCl4
    -induced acute liver injured
    mice and transiently expresses Anti-PEG Reporter on its surface. Second part of the research is tissues and organs transplantation research. We have generated Anti-PEG Reporter transgenic mice, and confirmed the mice genome DNA is, indeed, incorporated with Anti-PEG Reporter gene by genotyping. Then, we proved the Anti-PEG Reporter function by using both the PEG-modified protein and the PEG-modified probe. In this preliminary study, we have proven Anti-PEG Reporter’s properties, and expect its functions to be applied on tracking cell location and survival in future regenerative
    medicine and transplantation research.
    描述: 碩士
    指導教授：莊國祥
    資料類型: thesis
    顯示於類別:[生藥學研究所] 博碩士論文

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