| 摘要: | 本研究利用化學浸泡處理方法讓細胞黏附分子固定在鈦金屬植體表面上，以提高其與人牙周膜細胞（HPDLCs）的生物相容性。其做法為將鈦金屬植體浸泡在1%（V / V）p-vinylbenzoic acid solution 2小時後，再加入carboxyl groups在植體表面上。接著用去離子蒸餾水漂洗後，將鈦金屬植體浸入1.47%含有0.1 mg/ml Gly-Arg-Gly-Asp-Ser (GRGDS)，human plasma fibronectin (pFN), 或 type I collagen from calf skin (Col)的1-ethyl-3-(3-dimethylaminopropyl) carbodiimide solution，共價通過形成肽鍵(膠原固定)，可以固定在鈦植體表面上的細胞黏附分子。先前研究結果透過X射線光電子能譜分析，顯示細胞黏附分子成功地固定在鈦金屬表面上。另外掃描探針顯微鏡觀察可以得知膠原固定的鈦試片表面比起拋光的鈦試片表面具有更高的粗糙度，pFN固定與膠原固定後的鈦試片表面，其表面培養的細胞比起拋光表面具有較大程度的細胞展延現象(P < 0.05)，HPDLCs在膠原固定鈦試片表面顯示比拋光試片、GRGDS固定試片、pFN固定試片，可以讓粘著斑蛋白在細胞突起時更廣泛的展開與延續伸展。
其結果得知成功固定在鈦試片表面上的細胞粘附分子可以改善了與HPDLCs之間的生物相容性。此現象可以應用在牙科植體上，利用膠原固定鈦表面可形成類似圍繞韌帶組織，將此膠原固定後的類韌帶組織包覆植體後植入患處，可強化與細胞組織間的相容性。本研究將此化學浸泡處理方式搭配鈦金屬人工牙根，進行蘭嶼迷你豬口腔動物植牙試驗，在兩週與六週時間點犧牲動物分析其人工牙根骨接觸率與植體螺紋面積計算，其結果顯示膠原固定人工牙根在骨接觸率達81.12%，植體螺紋面積達92.22%。
The investigated use approach allow cell adhesion molecule fixed the surface of the titanium metal plant of chemical immersion, improve the with human periodontal ligament cells (HPDLCs) biocompatibility. The titanium dental implants were immersed in 1% (V / V) p-vinylbenzoic acid solution 2 hours, and added carboxyl groups on the surface of the implant. Then rinsing with deionized distilled water, titanium dental implants were immersed in 1.47% containing 0.1 mg / ml Gly-Arg-Gly-Asp-Ser (GRGDS), human plasma fibronectin (pFN), or type I collagen from calf skin (Col) of 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide solution, peptide bond formation by covalent (fixed by collagen ) can be fixed the cell adhesion molecule on the surface of a titanium dental implants.
Previous studies using X-ray photoelectron spectroscopy, showed that the cell adhesion molecules are immobilized on the titanium surface successfully. Scanning probe microscopy can see that the collagen fixed titanium specimen surface more roughness than polished titanium specimen surface, titanium specimen surface pFN fixed and collagen fixed, The cultured cells than the surface of the polishing surface having a greater degree of extension phenomena cells (P <0.05), HPDLCs wider expansion and continuation of the stretch when the vinculin in cell processes, display fixed in collagen titanium specimen surface than polished specimens, GRGDS fixed specimen, PFN fixed specimen.
Results that successfully fixed on the titanium surface of the specimen cell adhesion molecules can be improved between HPDLCs biocompatibility. This phenomenon can be applied to titanium dental implants, use of collagen fixed titanium surface may be formed similar organizations around the ligament, this type of collagen ligament tissue after fixation of the implant implantation after coating the affected area, can enhance compatibility between the cells and tissues.
In this study, treatment with titanium dental implants by this chemical immersion, orchid mini pigs were oral implant animals test, two weeks and six weeks sacrifice animals to analyze the titanium dental implants and bone contact rate implants threaded area calculation, the results are shown in bone collagen fixed artificial tooth root exposure rate of 81.12%, the explants threaded area of 92.22%. |
