探討次氯酸對人類肺上皮細胞的細胞毒性及粒線體活性之影響

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題名:	探討次氯酸對人類肺上皮細胞的細胞毒性及粒線體活性之影響 Effect of Hypochlorous Acid on Cytotoxicity and Mitochondrial Activity in Human Lung Epithelial Cells
作者:	劉亭妮 Liu, Ting-Ni
貢獻者:	保健營養學研究所
關鍵詞:	次氯酸 (hypochlorous acid、HOCl);人類呼吸道上皮細胞 hypochlorous acid (HOCl);human airway epithelial cell;cytotoxicity;mitochondrial biosynthesis and function
日期:	2015-11-30
上傳時間:	2021-01-07 10:57:54 (UTC+8)
摘要:	許多研究發現低濃度次氯酸 (hypochlorous acid, HOCl) 具有良好且廣泛之殺菌能力，目前已經逐漸運用在食品消毒殺菌、環境清潔並抑制病原菌孳生，以及甚至可以用在人體皮膚表面或傷口上的殺菌，幫助傷口的修復和癒合。雖然已證實次氯酸可以使用在表皮且不具刺激性，但是對於較脆弱並不具角質層之黏膜上皮組織的影響還是不確定的。鼻腔因為直接接觸環境的空氣，可能充滿了許多會造成過敏或呼吸道感染之病原體，因此若能有效減少其滯留與孳生在鼻腔中，則可以改善過敏的程度和感染的機會。本次研究即是針對次氯酸對人類呼吸道上皮細胞株 (A549及BEAS-2B) 的細胞毒性評估，觀察使用3.5-100 ppm和不同作用時間的次氯酸對細胞株的影響，與經常使用來殺菌消毒的次氯酸鈉 (sodium hypochlorite) 做比較，分析2者對於細胞活性和其粒線體生合成及功能的影響。結果發現相較於次氯酸鈉次氯酸的細胞毒性較低，3.5-60 ppm的次氯酸在短時間作用下不僅不會影響細胞活性和功能，甚至能進一步幫助細胞生長並促進粒線體活性，促使細胞增殖。 Many studies have found that low concentrations of hypochlorous acid (HOCl) has good and wide range of disinfection. Currently hypochlorous acid has been gradually used in food sterilization, environment cleaning and inhibition of pathogenic bacteria breeding, and could even be used on human skin or wound sterilization, help wound repaired and healing. Although it has been confirmed that hypochlorous acid couble be used without irritating the skin, but it is still uncertain that the cytotoxicity for the more vulnerable epithelium without stratum corneum. Direct contact with air environment, nasal cavity could be filled with many pathogens which can cause allergies or respiratory infection, so if effectively reduce pathogens retention and breeding in nasal cavity, maybe couble improve the degree of allergy and opportunity of infection. This study is the assessment of cytotoxicity against hypochlorous acid on human airway epithelial cell line (A549 and BEAS-2B). The results were analyzed for cell viability and mitochondrial biosynthesis and function. The effect of using different treatment time and 3.5-100 ppm of hypochlorous acid on cell lines was observed and compared with sodium hypochlorite (NaOCl) which often used for sterilization. These results showed that compared to sodium hypochlorite hypochlorous acid has low cytotoxicity on human airway epithelial cells. 3.5-60 ppm hypochlorous acid in short-term time treatment not only does not affect cell viability and function, and even further helps cell proliferation and promotes mitochondrial activity.
描述:	碩士指導教授:謝榮鴻委員:陳玉華委員:葉添順
資料類型:	thesis
顯示於類別:	[保健營養學系暨研究所] 博碩士論文

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