Taipei Medical University Institutional Repository:Item 987654321/5995
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    Please use this identifier to cite or link to this item: http://libir.tmu.edu.tw/handle/987654321/5995


    Title: Dexamethasone透過MKP-1訊息傳遞路徑抑制人類惡性神經膠質瘤細胞的侵襲性
    Dexamethasone Inhibits Cell Invasion of Human Malignant Glioma Cells via MKP-1
    Authors: 陶曉怡
    Hsiao-Yi Tao
    Contributors: 細胞及分子生物研究所
    Keywords: 膠質瘤
    侵襲性
    訊息傳遞
    內生性
    膠質瘤
    dexamethasone
    MK-1
    invasion
    Date: 2005
    Issue Date: 2009-09-11 15:50:07 (UTC+8)
    Abstract: Dexamethasone已經知道可以抑制癌細胞的侵襲性，但是其中的機制還不清楚。本研究探討人類惡性神經膠質瘤細胞中dexamethasone抑制細胞侵襲性的機制。人類惡性神經膠質瘤細胞具有內生性MMP-2的活性，且其活性與細胞的惡性度有關。p38 MAPK的抑制劑SB203580與p42/44 ERK的抑制劑PD98059都可以降低人類惡性神經膠質瘤細胞U87MG中MMP-2的活性。Dexamethasone可以誘發U87MG細胞中MKP-1的表現，所誘發的MKP-1隨著dexamethasone濃度的增加而增加，並隨著時間的增加而持續性表現。轉錄作用的抑制劑
    actinomycin D與轉譯作用的抑制劑cyclohexamide都可以阻斷U87MG細胞中dexamethasone所誘發MKP-1的表現，顯示dexamethasone所誘發MKP-1的表現是經由新蛋白的合成。RU486 (糖皮質激素受體拮抗劑)與triptolide (MKP-1阻斷劑)都可以降低U87MG細胞中dexamethasone所誘發的MKP-1，RU486可以逆轉dexamethasone所抑制的細胞侵襲性。NO donor sodium nitroprusside (SNP)可以逆轉U87MG細胞中dexamethasone所抑制MMP-2的活性。Triptolide可以逆轉U87MG細胞dexamethasone所抑制iNOS的活性。綜合以上結果可以得知，在U87MG細胞中，dexamethasone透過誘發MKP-1而抑制iNOS的表現及NO的釋出，進而抑制MMP-2的活性與細胞侵襲性。
    Dexamethasone has been shown to inhibit tumor invasion, however,
    the underneath mechanisms have not been elucidated. In the present study, we investigated the mechanism of which dexamethasone regulated the invasion in human malignant glioma cells. We found that human malignant glioma cells constitutively expressed MMP-2, which is well correlated with their malignancies. Treatment of glioma cells with SB203580 (a p38 MAPK inhibitor) or PD98059 (a p42/44 ERK inhibitor) reduced MMP-2 activity. Incubation of human malignant glioma cells with dexamethasone resulted in a dose- and time-dependent induction of a dual specific MAPK phosphatase, MAPK phosphatase-1(MKP-1). Treatment of glioma cells with actinomycin D (a transcription inhibitor) or cyclohexamide (a translation inhibitor) before the addition of dexamethasone decreased MKP-1 protein level, suggesting dexamethasone-induced MKP-1 expression required de novo protein synthesis. Pretreatment of cells with RU486 (a glucocorticoid receptor antagonist) or triptolide (a pharmacological MKP-1 blocker) reversed the inhibition of MMP-2 activity and cell invasion by dexamethasone. Treatment of glioma cells with NO donor, sodium nitroprusside (SNP), reversed the inhibition of MMP-2 activity by dexamethasone. In addition, pretreatment of triptolide reversed dexamethasone-reduced inducible nitric oxide synthase (iNOS) expression. Taken together, our data suggest that dexamethasone may inhibit tumor cell invasion and MMP-2 activity through MKP-1 induction, and that NO is the positive regulator of MMP-2 in human malignant glioma cells.
    Data Type: thesis
    Appears in Collections:[Graduate Institute of Medical Sciences] Dissertation/Thesis

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