飲食中不同n-6與n-3脂肪酸比例及蝦紅素對於誘發性口腔癌症之影響

Taipei Medical University Institutional Repository > 營養學院 > 保健營養學系暨研究所 > 博碩士論文 > Item 987654321/59459

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題名:	飲食中不同n-6與n-3脂肪酸比例及蝦紅素對於誘發性口腔癌症之影響 Effects of Various Dietary n-6/n-3 Fatty Acids and Astaxanthin on Induced Oral Carcinoma
作者:	梁安 Liang, An
貢獻者:	黃士懿
關鍵詞:	口腔癌;檳榔嚼塊;n-6/n-3脂肪酸比例;蝦紅素;腫瘤負荷 oral carcinoma;betel quid;dietary fatty acids;astaxanthin;tumor burden
日期:	2015-06-26
上傳時間:	2020-08-28 14:47:07 (UTC+8)
摘要:	根據流病統計的結果發現，口腔癌的發生率日益上升，且主要發生在台灣、印度等有嚼食檳榔嚼塊習慣的東南亞國家。嚼食檳榔時，會因檳榔嚼塊中之化學成分及其粗纖維造成口腔黏膜發炎並增加其氧化壓力，造成損傷。長期嚼食檳榔將導致口腔癌症之發生。過去的研究指出，降低發炎反應與氧化壓力皆可能是預防或是治療癌症的方式。文獻指出，飲食中的脂肪酸組成可能與某些特定癌症的發生有關，改變飲食中脂肪酸之組成可能可以降低特定癌症之發生率；另有文獻顯示，類胡蘿蔔素具有強大的清除自由基之能力，並可調控細胞週期達到癌症化學預防之功效。故本研究利用7,12-dimethylbenz[a]anthracene(DMBA)及檳榔嚼塊萃取物來誘發倉鼠口腔癌前病變，透過調整實驗飼料中n-6及n-3脂肪酸的比例且在不同的n-6/n-3飲食下以塗抹的方式給予蝦紅素，以探討是否能夠降低實驗動物體內發炎反應與氧化壓力，達到預防或是改善口腔癌之發生。研究設計使用45隻6週齡之雄性倉鼠，依照實驗飼料的不同分為正常(N組，n-6/n-3=6)、低(L組，n-6/n-3=2)與高(H組，n-6/n-3=15) n-6/n-3脂肪酸飲食，並依照不同的塗抹物質各自再細分為正常組(N)、負控制組(C)及蝦紅素組(A)。除了正常組(NN、LN及HN)外，其餘的六組(NC、LC、HC、NA、LA及HA)於實驗第1週至第5週會使用0.5% DMBA(溶於礦物油中)塗抹於倉鼠右側口腔頰囊，以誘發其口腔癌化，在實驗第6至16週時則以檳榔嚼塊萃取物(betel quid extract, BQE)取代0.5% DMBA進行塗抹。蝦紅素組(NA、LA及HA)則在實驗第6至16週時除了塗抹BQE外，同時以0.5%蝦紅素(溶於礦物油中)與BQE隔日交互塗抹於右側口腔頰囊。結果發現，給予倉鼠低n-6/n-3脂肪酸飲食(n-6/n-3=2)可以降低其體內n-6/n-3脂肪酸比例，向下調節口腔頰囊NF-κB、cyclin D1之表現量，並減少細胞增生指標─細胞增殖核抗原(proliferating cell nuclear antigen, PCNA)之表現量，達到顯著減少口腔腫瘤數目、腫瘤體積及腫瘤負荷。然而，無論在何種n-6/n-3脂肪酸飲食下，輔以塗抹0.5%之蝦紅素於具口腔癌前病變之倉鼠，均無法有效減緩其口腔癌前病變之發生。 Betel quid (BQ) chewing is thought to contribute to leukoplakia, submucosal fibrosis and oral cancer in Taiwan, India and many Southeast Asian countries. Dietary fatty acid patterns have been linked to certain cancer prevalence. Carotenoids (i.e. astaxanthin) have been indicated as possessing a strong ability to protect cells against active oxygen species by interfering with protein expression in the cell cycle. Thus, we investigated the chemopreventive effects of various dietary n-6/n-3 fatty acid and astaxanthin under DMBA/Betel quid extract (BQE)-induced hamster oral cancer model. Forty-five 6-week-old adult male hamsters were housed and divided into normal (N), low (L) and high (H) dietary n-6/n-3 fatty acid groups and subdiveid into normal (N), control (C) and astaxanthin (A) groups (n=5 for each subgroup). All animals except normal group were initially daubed with 0.2 mL of 0.5% DMBA solution to the right buccal pouch of animals for 5 weeks. In the subsequent 11 weeks, these hamsters were daubed with either BQE or a mineral oil solution 0.2 mL of 0.5% astaxanthin three times per week on alternate days. Results indicate the low dietary n-6/n-3 fatty acid group presented the significant lower tumor number, volume and tumor burden than other groups. Furthermore, the low dietary n-6/n-3 fatty acid group presented the significant lower NF-κB, cyclinD1 and PCNA expression in the right buccal pouch tissue and lower n-6/n-3 fatty acids profile in red blood cell than other groups. In conclusion, lower dietary n-6/n-3 fatty acids showed the chemopreventive effects on DMBA/BQE-induced hamster oral cancer model. However, our results indicated astaxanthin lack of chemopreventive effects under various n-6/n-3 fatty acids diets on DMBA/BQE-induced hamster oral cancer model.
描述:	碩士指導教授:黃士懿委員:林永和委員:楊素卿
資料類型:	thesis
顯示於類別:	[保健營養學系暨研究所] 博碩士論文

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