| 摘要: | 本研究分為兩部份，第一部份主要探討粟米草科（Molluginaceae）植物假繁縷Glinus oppositifolius全草甲醇萃取物之活性成分探討。假繁縷全草甲醇萃取物經由各種層析技術，共分離出十六個化合物，根據核磁共振光譜分析、物理數據及文獻資料比對後，其結構分別確定為：kaempferol (1)、kaempferol 3-O-β-D-glucopyranoside (2)、kaempferol 3-O-β-D-galactopyranoside (3)、vicenin II (4)、p-hydroxybenzoic acid (5)、vanillic acid (6)、trans-ferulic acid (7)、trans-cinnamic acid (8)、p-hydroxyacetophenone (9)、vanillin (10)、lotoidoside E (11)、spergulin A (12)、spergulacin (13)、β-sitosterol (14)、spinasterol 3-O-β-D-glucopyranoside (15)及adenosine (16)，其中化合物2、11、15則從假繁縷中為首次被分離出來。另外，第二部份進行菊科（Asteraceae）植物扁桃斑鳩菊Vernonia amygdalina葉子甲醇萃取物之活性成分。扁桃斑鳩菊葉部醇萃取物經由各種層析管柱及不同溶媒系統層析後，共分離出十二個化合物，根據核磁共振光譜分析、物理數據及文獻資料比對後，其結構分別確定為：L-phenylalanine (17)、uracil (18)、(R)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (19)、(Z)-2-(4-hydroxybenzyl)butenedioic acid (20)、p-hydroxyphenyl itaconic acid (21)、vernonioside B2 (22)、vernonioside B5 (23)、chlorogenic acid (24)、1,3-O-dicaffeoylquinic acid (25)、methyl 3,5-O-dicaffeoylquinate (26)、luteolin (27)及luteolin-7-O-β-D-glucopyranoside (28)。其中化合物17, 18為由扁桃斑鳩菊中首次被分離出來，而化合物19, 21為首次從天然物中分離出來，化合物23則為新化合物。利用小鼠肝臟細胞（FL83B）葡萄糖攝取，評估經純化後之化合物活性，化合物濃度2.5 µM下，假繁縷分離之化合物4 (101.3%)、11 (104.1%)、12 (124.1%)具有促進效果，扁桃斑鳩菊葉部分離之化合物17 (111.0%)、20 (106.4%)、25 (104.3%)有促進作用。
This study divided into two parts. Part one aimed to investigate the active ingredients of the MeOH extract of Glinus oppositifolius, which belongs to Molluginaceae. Sixteen compounds were isolated via various chromatographic techniques and their structures were further identified according to their 1D, 2D NMR spectroscopy and physical data. They are kaempferol (1), kaempferol 3-O-β-D-glucopyranoside (2), kaempferol 3-O-β-D-galacto-pyranoside (3), vicenin II (4), p-hydroxybenzoic acid (5), vanillic acid (6), trans-ferulic acid (7), trans-cinnamic acid (8), p-hydroxyacetophenone (9), vanillin (10), lotoidoside E (11), spergulin A (12), spergulacin (13), β-sitosterol (14), spinasterol 3-O-β-D-glucopyranoside (15), adenosine (16). Among them, the isolation of compound 2, 11 and 15 from Glinus oppositifolius are the first report.
Part two aimed to investigate the active ingredients of the MeOH extract of Vernonia amygdalina, which belongs to Asteraceae. Twelve compounds were isolated via column chromatography with various solvent systems and their structures were further identified according to their 1D, 2D NMR spectroscopy and physical data. They are L-phenylalanine (17), uracil (18), (R)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (19), (Z)-2-(4-hydroxy-benzyl)butenedioic acid (20), p-hydroxyphenyl itaconic acid (21), vernonioside B2 (22), vernonioside B5 (23), chlorogenic acid (24), 1,3-O-dicaffeoylquinic acid (25), methyl 3,5-O-dicaffeoylquinate (26), luteolin (27), luteolin-7-O-β-D-glucopyranoside (28). Among them, compound 17, 18 are the first report from Vernonia amygdalina. Additionally, the obtained compound 19, 21 to be found from natural resources are the first case and compound 23 is new compound.
Among the isolated compounds from Glinus oppositifolius, 2.5 µM of compounds 4, 11, 12 were demonstrated to enhance glucose uptake with 101.3%, 104.1%, 124.1%, respectively, using a mouse liver cell FL83B. And the isolated compounds from Vernonia amygdalina, 2.5 µM of compounds 17, 20, 25 were demonstrated to enhance glucose uptake with 111.0%, 106.4%, 104.3%, respectively, using a mouse liver cell FL83B. |
