影響電阻抗法製作之全血凝固傳感器參數探討

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題名:	影響電阻抗法製作之全血凝固傳感器參數探討 Discussion on Parameters of Whole Blood Coagulation Sensors Produced by Electrical Impedance Method
作者:	陳宣惟 Chen, Hsuan-Wei
貢獻者:	生醫光機電研究所黃豪銘馮聖偉
關鍵詞:	AD5933阻抗量測;電化學阻抗;頻率響應;相位;溫度對凝血時間影響 AD5933 impedance measurement;Electrochemical Impedance Spectroscopy;Frequency response;Phase;Temperature effect on clotting time
日期:	2019-06-19
上傳時間:	2020-02-18 12:40:59 (UTC+8)
摘要:	血液是在動物的循環系統、心臟和血管腔內循環流動的一種組織，可以將氧氣及營養素送到各器官，並將細胞的代謝廢棄物帶離細胞。當人體受傷時，身體會產生相對應的反應以避免大量出血，血液凝固是一個複雜、動態的生理過程，血液會在受傷的地方凝固並止血，由液體狀態逐漸轉變為不流動的凝膠狀態，是生理性止血的重要環節。然而常規凝血測定方法在臨床應用無法在極短的時間內即時提供結果，對於重症患者而言，即時檢驗已變得越來越有吸引力，因為它們不僅比臨床實驗室檢測產生更快的結果，而且它們還可以提供關於止血系統狀況的更完整的訊息。本論文提出電阻抗分析法以檢測所設計之晶片，該儀器具有低功耗、低成本、輕巧化等方面優勢且使用方便。所設計之晶片透過表面塗層塗覆助凝劑，縮短凝血檢測時間，並探討不同環境溫度對凝血時間之影響。本研究並以AD5933阻抗分析晶片做為小型化儀器開發之晶片，在量測待測物前先經過量測已知電阻取得增益係數，並製備以壓克力(PMMA)為基材之檢測晶片，使用氧電漿對PMMA表面進行改質，以不鏽鋼遮罩覆蓋於此檢測晶片，透過濺鍍與Lift-off技術完成晶片電極。而用於檢測血液之電極處表面經過噴砂處理達成粗糙化需求以提供血液樣品更佳的附著度。量測環境溫度自25 °C開始每隔5°C測量，輔以不同濃度之氯化鈣溶液觀察對於凝血之影響。初步結果顯示本研究系統架構在環境溫度45°C下，使用100Ω做為AD5933量測晶片回饋電阻時，其誤差值最小最適合做為回饋電阻，使用此晶片作為分析儀進行全血凝固量測可觀察到相位變化，然而於阻抗方面並無顯著變化，且於相位量測圖並無觀察到高低頻率之特徵點。關鍵字: AD5933阻抗量測、電化學阻抗、頻率響應、相位、溫度對凝血時間影響 Blood is a tissue that circulates in the animal's circulatory system, heart and blood vessels. It can send oxygen and nutrients to various organs and carry the metabolic waste of cells away from the cells.When the human body is injured, the body will respond accordingly to avoid massive bleeding. Blood coagulation is a complex and dynamic physiological process. The blood will coagulate and stop bleeding in the injured area, and gradually change from a liquid state to a non-flowing gel state. It is an important part of physiological hemostasis. However, conventional coagulation assays do not provide immediate results in clinical applications in a very short period of time. For critically ill patients, immediate testing has become increasingly attractive because they produce not only faster results than clinical laboratory testing and they can also provide a more complete message about the condition of the hemostatic system. This paper proposes an electrical impedance analyzer based on AD5933 to detect the designed wafer. The instrument has the advantages of low power consumption, low cost, light weight and is convenient to use. The designed wafer is coated with a coagulant through a surface coating to shorten the time of coagulation detection and to investigate the effect of different ambient temperatures on clotting time. In this study, the AD5933 impedance analyzer was used as a wafer developed for miniaturized instruments. Before measuring the object to be tested, the gain coefficient was obtained by measuring the known resistance, and a test wafer based on acrylic (PMMA) was prepared. The PMMA surface was modified with an oxygen plasma, covered with a stainless steel mask, and the wafer electrode was completed by sputtering and Lift-off techniques. The surface of the electrode for detecting blood is subjected to sandblasting to achieve a roughening requirement to provide better adhesion of the blood sample. The measured ambient temperature was measured at 5 ° C starting at 25 ° C, and the effect of coagulation was observed with different concentrations of calcium chloride solution. It is initially shown that when using 100Ω as the AD5933 measurement chip feedback resistor, the minimum error value is most suitable as the feedback resistance. The phase change can be observed by using the wafer as an analyzer for whole blood coagulation measurement. However, there was no significant change in impedance, and no characteristic points of high and low frequencies were observed in the phase measurement chart. Key words: AD5933 impedance measurement; Electrochemical Impedance Spectroscopy; Frequency response; Phase; Temperature effect on clotting time.
描述:	碩士指導教授：黃豪銘共同指導教授：馮聖偉委員：陳祥和委員：楊自森委員：劉健群
資料類型:	thesis
顯示於類別:	[生醫光機電研究所] 博碩士論文

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