The Application of Proteomic Analysis in Gastric Cancer Plasma Protein Biomarker Discovery

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題名:	The Application of Proteomic Analysis in Gastric Cancer Plasma Protein Biomarker Discovery
作者:	Yudha Nur Patria
貢獻者:	林裕峯
關鍵詞:	Gastric cancer;Mass spectrometry;Proteomics;SHBG;Protein biomarkers
日期:	2015-01-12
上傳時間:	2020-01-13 12:26:22 (UTC+8)
摘要:	Background: Gastric cancer is the third most common cause of cancer death after lung and liver cancer which causes approximately 800,000 deaths in 2012. Development of plasma biomarkers into screening, diagnostic, prognostic and treatment monitoring tool has shown promising results in improving the management strategy of certain types of cancer. However, in gastric cancer, the use of plasma biomarkers is limited. Therefore, this study is aimed to discover novel plasma protein biomarkers for gastric cancer which may be further developed into screening, diagnostic, prognostic and treatment monitoring tools. Methods: Blood plasma from 24 gastric cancer patients and 9 healthy controls were collected from TMU Hospital Biobank and were prepared for LC-MS/MS mass spectrometry assay. We further analysed the data obtained from mass spectrometry assay using PEAKS7 bioinformatic software to identify potential candidates of plasma protein biomarkers. Verification and validation of the identified biomarker candidates in different patient cohort were conducted using immunoblotting assay. Results: We found 4 potential plasma protein biomarker candidates; APOC-1, gelsolin, SHBG and CO4A which were significantly overexpressed in gastric cancer patients. Validation study using immunoblotting assay found only SHBG showed a consistent result in which the plasma expression was significantly higher in patient group (p<0.01). Area under the curve of the ROC curve for SHBG expression was 0.89 (95% CI 0.74-1.00, p<0.01). Conclusion: LC-MS/MS mass spectrometry assay is a promising tool for screening of blood plasma gastric cancer biomarkers. The plasma SHBG level has a potential as a gastric cancer biomarker and is worthy for further investigation.
描述:	碩士指導教授-鄭朝文共同指導教授-林裕峯委員-林景堉委員-趙載光委員-廖辰中
資料類型:	thesis
顯示於類別:	[醫學科學研究所] 博碩士論文

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