添加不同比例P/S比值及不同含量的單元不飽和脂肪酸對 3T3-L1細胞脂質生合成之影響

Taipei Medical University Institutional Repository > 營養學院 > 保健營養學系暨研究所 > 碩博士論文 > Item 987654321/58095

請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/58095

題名:	添加不同比例P/S比值及不同含量的單元不飽和脂肪酸對 3T3-L1細胞脂質生合成之影響 Effects of P/S ratio and different amount of monounsaturated fatty acids on adipogenensis in 3T3-L1 cells
作者:	林心怡 Lim, Sim Yee
貢獻者:	簡怡雯
關鍵詞:	多元不飽和脂肪酸與飽和脂肪酸比值;單元不飽和脂肪酸;脂肪細胞;脂質生合成作用 polyunsaturated fatty acid to saturated fatty acid ratio;monounsaturated fatty acid;adipocytes;lipid metabolism
日期:	2014-07-04
上傳時間:	2019-09-24 12:49:45 (UTC+8)
摘要:	本篇研究目的為探討高不飽和脂肪酸與飽和脂肪酸比值 (P/S ratio) 搭配不同含量的單元不飽和脂肪酸對3T3-L1脂肪細胞脂質生合成及脂質相關代謝之作用機轉。3T3-L1前脂肪細胞先培養於含dexamethasone、3-isobutyl-1-methylxanthine及insulin的Dulbecco’s modified eagle medium (DMEM) 培養液中，再以含insulin的培養液培養使其分化為成熟脂肪細胞後，分別用體積比1: 10: 5及1: 2.5: 5的方式混合棕櫚酸、油酸及亞麻油酸培養脂肪細胞 (相等於P/S ratio=5; MUFA 60%，簡稱M60及P/S ratio=5; MUFA 30%，簡稱 M30) 。經過72小時後，以油紅染色分析脂肪酸對細胞內脂質堆積程度的影響，並分析細胞內三酸甘油酯 (TG) 的含量; 同時分析培養基中甘油的釋出量以及利用real-time PCR的方法分析脂質代謝相關基因peroxisomal proliferator-activated receptor γ (PPAR γ) 、leptin、adiponectin、glucose transporter-4 (GLUT-4) 、hormone-sensitive lipase (HSL) 及lipoprotein lipase (LPL) 的表現。結果發現，10 μM低劑量的M60組 (M60-10) 與同劑量的M30組 (M30-10) 相比，可顯著降低細胞內TG含量以及PPAR γ、LPL、leptin、adiponectin、GLUT-4 mRNA的表現量，同時能增加脂解作用相關酵素HSL mRNA的表現，進而達到降低脂肪細胞三酸甘油脂蓄積的效果。 The development of obesity is characterized by an increase in the number of fat cells and their lipid content, as a result of adipogenesis and differentiation. In this study, we determined the effects of high polyunsaturated to saturated fatty acid ratio (P/S ratio=5) and different amount of monounsaturated fatty acid (MUFA=30% or 60%) on adipogenesis in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were cultured in differentiated medium for 12 days to differentiate into mature adipocytes. Different ratio of palmitic acid (PA), oleic acid (OA) and linoleic acid (LA) will first bound with bovine serum albumin (BSA) before given to 3T3-L1 adipocytes in low dose and high dose. Results showed that low dose of P/S ratio=5, MUFA=60% (M60) fatty acid could decrease the accumulation of triglycerides in mature adipocytes by decreasing the mRNA expression of adipogenic factors such as peroxisome proliferator-activated receptors (PPARs) , lipoprotein lipase (LPL) and glucose transporter-4 (GLUT-4) whereas the lipolytic enzymes (hormone sensitive lipase, HSL) expression was increased. Furthermore, the treatment of M60 in low dose also decreased the secretion of leptin and increased the secretion of adiponectin in adipocytes. In summary, the composition of P/S=5, MUFA 60% fatty acid in low dose seems to have anti-adipogenic effects on 3T3-L1 adipocytes due to the down-regulation of adipogenic effects and transcription factors.
描述:	碩士指導教授-簡怡雯委員-楊素卿委員-黃惠宇
資料類型:	thesis
顯示於類別:	[保健營養學系暨研究所] 碩博士論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	399	檢視/開啟

檢視Licence

在TMUIR中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....