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    題名: 年齡對脂肪幹細胞之骨誘導分化影響
    The age-dependent osteogenic differentiation of adipose-derived stem cells
    作者: 曾富宏
    Tseng, Fu-Hung
    貢獻者: 生醫材料暨工程研究所
    鄧文炳
    吳駿翃
    關鍵詞: 脂肪間葉幹細胞;骨再生作用;骨分化;骨質疏鬆症;早老化老鼠
    adipose-derive stem cells (ADSCs);bone formation;osteogenic differentiation;osteoporosis;senescence accelerated mice (SAM)
    日期: 2010-06-30
    上傳時間: 2019-06-12 09:19:39 (UTC+8)
    摘要: 本研究目的，是探討年齡對脂肪間葉幹細胞(adipose-derive stem cells, ADSCs)之骨生成作用影響。基於先前實驗室建立之血小板濃厚液(platelet rich plasma)促使骨前驅細胞其增生及骨分化能力，再移植至去卵巢早老化老鼠(OVX-SAMP8)中，進行骨質疏鬆之預防與治療研究，此結果已發表至Journal of Nuclear Medicine國際期刊上。故本實驗，鑒於先前建立細胞移植模式外，將深入探討不同年齡ADSC於體外及骨鬆老鼠內之骨誘導分化影響。首先第一部分體外實驗，於早老化老鼠腹腔中，分別取得年輕族群（1月齡）與老年族群（10月齡）之脂肪幹細胞進行體外定性實驗，並試驗出最適生長環境，以利維持不同年齡之幹細胞活性，實驗結果證實年輕族群之脂肪幹細胞其增生速率(proliferation rate)、自我複製(self-renewal)能力及細胞骨誘導分化表現，皆優於老年族群之脂肪幹細胞。第二部分動物實驗，將延續體外試驗，進而應用於骨鬆動物中，探討不同年齡的脂肪幹細胞來源於體內環境中，是否影響其骨生成作用。骨質密度結果發現，此治療模式可修復骨質流失現象並優於老年族群脂肪幹細胞之骨修復能力。該兩大部分實驗之建立，有助於現今細胞治療中，提供不同年齡幹細胞給予受試者之臨床前發展。
    The aim of this thesis is to study the effects of aging on the ability of bone formation in ADSCs. Previously, we have established a novel therapeutic approach based on transplantation with progenitor cells (NIH3T3), in order to prevent osteoporosis and prolong the lifespan of the ovariectomized-senescence-accelerated mice (OVX-SAMP8). We decided to further our previous study by determining the aging effect on osteogenic differentiation potential of ADSCs. ADSCs were isolated from adipose tissues which obtained from female SAMP8 mice with different ages for in vitro study. First, we used flow cytometry to characterize the isolated cells by examining the expression of surface markers such as CD45, CD34, Sca-1, CD44 and CD105. Second, we compared the aging effects on the growth kinetics and differentiation potential of ADSCs between young (one month old) and old (ten month old) from female SAMP8 mice, and found there was a significant difference in both the proliferation rate and osteo-differentiation potential. Differentiated ADSCs were transplanted into the bone marrow in osteoporotic mice (OVX-SAMP8). Therefore, transplantion of differentiated ADSC cells was shown effective in restoring bone mineral density from the right/left knees and femurs at 3 months and the differentiated young group (ADSC-1M) showed significantly higher bone regeneration in OVX-SAMP8 mice, comparing to the differentiated old group (ADSC-10M). In conclusion, these findings provided important insights on emerging cell-based therapeutic strategies, especially in the treatment of various bone disorders including osteoporosis.
    描述: 碩士
    指導教授-鄧文炳
    委員-吳志雄
    委員-林峰輝
    委員-黃效民
    委員-黃彥華
    共同指導教授-吳駿翃
    資料類型: thesis
    顯示於類別:[生醫材料暨組織工程研究所] 博碩士論文

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