Taipei Medical University Institutional Repository:Item 987654321/5760
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    Title: 松杉靈芝三萜類純化及對小白鼠急性肝障礙排除之影響
    Isolation of triterpenoids from Ganoderma tsugae and their effect on acute-hepatotoxicity in mice
    Authors: 詹美華
    CHAN, MEI-HUA
    Contributors: 天然物醫學研究所
    Keywords: 急性肝障礙
    松杉靈芝
    純化
    Date: 1991
    Issue Date: 2009-09-11 15:42:13 (UTC+8)
    Abstract: 松杉靈芝子實體95﹪酒精萃取物,溶於氯仿,用飽和碳酸氫鈉水溶液分配萃取,取
    飽和碳酸氫鈉水溶液層於 0℃加6N鹽酸,在PH值=3時收集沈澱物乾燥,並用少量氯
    仿溶解,經矽膠 (200g) 管柱色層分析,順序以氯仿、甲醇比為 98:2,9:1,1:1
    及完全甲醇為移動相初步純化,再用高效液態色層分析法,製備用色層分析法,再
    結晶等方法,得到4種三類,經UV、IR、H-NMR、C-NMR、mass等光譜分析鑑
    定,分別是 GT-1:lucidon A,GT-2:lucidenol,GT-3:ganoderic acid C2 ,
    GT-4:ganoderic acid B。其在薄層色層分析法中,以10﹪硫酸呈色為紅色。
    在動物生理活性實驗方面,用四氯化碳誘發之急性肝障礙為模式,以小白鼠血清中
    GPT 和GOT 的值為評估標準。第一部份,以 Silymarin、GT-1、GT-2、GT-3和GT-4
    當餵食藥品,結果顯示,GT-4有抑制小白鼠以四氯化碳誘發之急性肝障礙的功用。
    第二部份,以不同劑量的GT-4為餵食藥品,結果顯示,不同劑量的GT-4有類似之效
    果。另外,由實驗結果得到,降低小白鼠之血清GPT 和GOT 值效果最顯著的是:每
    公斤老鼠體重餵食0.4mg 的GT-4。
    Data Type: thesis
    Appears in Collections:[Graduate Institute of Medical Sciences] Dissertation/Thesis

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