Taipei Medical University Institutional Repository:Item 987654321/5588
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 45069/58245 (77%)
造访人次 : 2369151      在线人数 : 164
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/5588


    题名: 長期給予懷孕母鼠嗎啡對其所生幼鼠學習與記憶之影響及其分子機制
    The effect of prenatal exposure to morphine on learning and memory and activation of CREB & PKC in the hippocampus of developing rats
    作者: 魏妏純
    Wei Wen Chun
    贡献者: 醫學研究所
    关键词: 嗎啡
    蛋白質激酶
    C
    海馬迴
    學習與記憶
    morphine
    PKC
    hippocampus
    learning and memory
    日期: 2003
    上传时间: 2009-09-11 15:37:03 (UTC+8)
    摘要: 中文摘要
    在過去臨床研究發現,懷孕期間施打嗎啡或海洛英成癮的母親所生下的小孩,他們的學習能力以及社會適應比一般正常的孩子來的差,其神經病裡機轉仍未清楚。之前我們實驗室已經證實,在懷孕期間注射嗎啡的母鼠,所生之幼鼠的發育過程中,其海馬迴裡的N-methyl-D-asparate (NMDA) receptor以及經由kainic acid所誘發之calcium/calmodulin kinase II(CaMKII)的活性,會顯著比對照組之幼鼠低。如果NMDA receptor和CaMKII的活化,對學習與記憶的能力很重要,那麼懷孕期接觸嗎啡所生幼鼠其學習與記憶能力的發展有可能受影響。因此為了證實上述的論點,我們以水迷宮的實驗,來偵測懷孕期間注射嗎啡的母鼠,所生之幼鼠其學習與記憶的能力。並且以西方墨點法來測量海馬迴中,兩個與學習記憶相關的蛋白質活化型態即活化型PKC和CREB的表現量。水迷宮的實驗結果顯示在出生後第30天,嗎啡組和控制組幼鼠在學習與記憶的能力上沒有什麼差異。但在出生後第60天,嗎啡組的能力都比控制組差。而西方墨點法的結果顯示在出生後第60天,嗎啡組的內生性活化型CREB明顯的比控制組低。另一方面,出生後第30天和第60天,嗎啡組和控制組在活化型PKC的表現上沒有差異。此外,在所有的實驗老鼠中,嗎啡組和控制組在經由KA所誘導活化的CREB和PKC的表現量,皆無顯著差異,。本篇研究推測發育中的幼鼠在出生以前即受到嗎啡的影響,會降低其學習與記憶的能力。並推測這個結果是由於海馬迴中NMDA receptor-CaMKII-CREB此一路徑的活化所受到抑制而造成。
    英文摘要
    Certain neuropsychological sequels, particularly in the ability of learning and social adaptation, have found in children born to mothers addicted to morphine or heroin during pregnancy. The underlying neuropsychological mechanisms we unclear. We, previous had found that rats born to dams rats chronically received daily morphine injection through the whole course of pregnancy had decrease in the expression of N-methyl-D-asparate (NMDA) receptor and kainic acid-induced calcium/calmodulin kinase II activity in the hippocampus during developing stage. Given that activation of the NMDA receptor and CaMKII are essentially required for the function of learning and memory, it is possible that that prenatal exposure to morphine will cause disability in the learning and memory in the developing rats born to morphine-treated rats. To address this issue we determine the ability of learning and memory in the rats born to dam rats chronically received morphine during prenatal period by water maze experiment. We also determined the expression of two elements downstream to the activation of NMDA receptor and CaMKII, namely, the activation of protein kinase C and cAMP response element binding protein (CREB), in the hippocampus using immunoblotting assay. The study of water maze showed that no change in the ability of learning or memory in the morphine group rats at postnatal day 30 as compared to that of control group rats. However, there is a trend of decrease in both learning and memory ability of morphine group rats on postnatal day (PND)60. Immunoblotting assay study showed that on PND 30 and 60, the endogenous activated form of CREB of morphine was significantly lower than that of control group rats. On the other hand, no difference in the expression of activated form of PKC between control and morphine group rats on either PND30 or PND 60. There is no difference between control and morphine group in term of kainic acid-induced activation of CREB and PKC on any examined PND. This study suggests that prenatal exposure to morphine might disturb the development of learning and memory function in the developing rats, and one possible mechanism is through the inhibition in the activation of NMDA receptor-CaMKII-CREB pathway in the hippocampus.
    数据类型: thesis
    显示于类别:[醫學科學研究所] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    摘要.doc34KbMicrosoft Word74检视/开启
    摘要.pdf64KbAdobe PDF102检视/开启
    摘要.ppt138KbMicrosoft Powerpoint52检视/开启
    摘要.ps371KbPostscript53检视/开启


    在TMUIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    著作權聲明 Copyright Notice
    • 本平台之數位內容為臺北醫學大學所收錄之機構典藏,包含體系內各式學術著作及學術產出。秉持開放取用的精神,提供使用者進行資料檢索、下載與取用,惟仍請適度、合理地於合法範圍內使用本平台之內容,以尊重著作權人之權益。商業上之利用,請先取得著作權人之授權。

      The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.

    • 瀏覽或使用本平台,視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例,並不得為任何不法目的使用TMUIR。

      By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.

    • 本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者,煩請權利人通知本平台維護人員([email protected]),將立即採取移除該數位著作等補救措施。

      TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

    Back to Top
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈