研究茶成份調控尼古丁受體作用於單核球引起動脈硬化及其未來臨床應用

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Title:	研究茶成份調控尼古丁受體作用於單核球引起動脈硬化及其未來臨床應用 Investigate the roles of Tea extract through modulating neuro-Nicotine Acetylcholine receptor on monocytes involved in atherosclerosis and it's potential clinical application
Authors:	何梓豪 Ho, Chi-Hou
Keywords:	尼古丁受體;Nicotine Receptors
Date:	2013-06-07
Issue Date:	2018-11-16 12:48:24 (UTC+8)
Abstract:	近年來腦血管疾病、糖尿病、高血壓疾病與抽煙被證實會增加冠狀動脈疾病的風險，而在罹患動脈粥狀硬化病人之血管斑塊中，發現大量泡沫細胞(單核球為其前驅物)之累積是動脈粥狀硬化發生的誘因之一。然而，煙害與泡沫細胞之形成機制目前尚未明朗。因此本研究中我們希望釐清尼古丁(香煙之主要成分)與泡沫細胞形成之交互關係，而天然物之添加是否可抑制泡沫細胞之形成進而達到預防動脈硬化之作用。我們發現人類單核球能高表現尼古丁受體─α9-nAchR(相較於其他種類之白血球)，而尼古丁環境之曝露能增加其表現量及泡沫細胞之形成。為了進一步釐清尼古丁受體在煙害與泡沫細胞之形成所扮演之角色，以及尋找有效減緩煙害之天然物，根據先前之研究成果發現兒茶素(茶中成份)能有效抑制α9-nAchR之表現以及癌細胞之生長作為基礎，啟發我們進行人體飲用普洱茶試驗。我們發現飲用普洱茶能有效降低各受試者體內單核球α9-nAchR之表現量以及減少煙害誘發之泡沫細胞。此外，抽煙者試驗前期單核球α9-nAchR之表現量以及曝露於煙害環境而誘發之泡沫細胞皆高於非吸煙者。從統計分析結果發現，服用普洱茶膠囊後能有效改善人體多項生化指標之表現，進而改善受試者身體之健康狀況。為了印證人體試驗之研究成果，我們利用紅茶、綠茶之粗萃取物進行細胞實驗，我們發現兩種茶萃取物皆能抑制單核球上α9-nAchR表現以及減少泡沫細胞形成。說明茶中皆具有某些活性成份(如：EGCG)能減緩煙害對於單核球之影響，進而減少泡沫細胞形成。本研究發現，尼古丁環境之曝露能刺激尼古丁受體表現及誘導泡沫細胞之形成，而此反應能受到茶中活性成份所抑制。然而，尼古丁受體與泡沫細胞形成之機轉還需繼續釐清。在未來應用層面上，我們希望進一步證實茶中活性成份能有效減緩煙害以及泡沫細胞之形成，進而推廣喝茶之好處以及臨床上之應用。 Recently, stroke, diabetes, high blood pressure and smoking were proved to be the major risk factors of cardiovascular diseases. There were a large number of foam cells which were formed by monocytes, found in the atheroma plaques of atherosclerosis patients. Though, the tobacco and foam cell formation mechanism is not yet apparent, which anticipate us to make clear of nicotine (cigarettes is the major component) and foam cell formation interactive relationship, and whether the addition of natural products would inhibit the formation of foam cells and as a result to achieve the prevention of atherosclerosis. We investigate that human monocytes could be high performance in nicotine receptor ─ α9-nAchR (in comparison to other types of white blood cells), and nicotine exposure to environment can increase the performance of its volume and foam cell formation. Previous research found that catechins (tea ingredients) could effectively reduce the performance of α9-nAchR and the growth of cancer cells, hence, we are inspired to clarify that tobacco and nicotine receptors in the formation of foam cells and the function played, as well as to discover the effective mitigation of natural products of tobacco; therefore, human trials drinking tea was preformed. We found that drinking tea can effectively reduce the α9-nAchR performance of each individual monocyte and the amount of tobacco-induced foam cell. In addition, theα9-nAchR monocytes expression and exposure to tobacco environment induced foam cell in the pre-trail smokers, which were higher than non-smokers. From the statistical results, taking tea capsules can effectively improve number of biochemical parameters, thereby improving the health status of the human body. In order to prove human trials and study results, we make use of black tea, green tea extract for cell crude experiments, and discover that two kinds of tea extracts inhibitα9-nAchR monocyte performance and reduce foam cell formation. All tea have certain active ingredients (eg: EGCG), which can slow down the monocytes, thereby reducing foam cell formation. The study investigate that nicotine exposure to environment can stimulate nicotinic receptor expression, hence, inducing the formation of foam cells. This reaction can be inhibited by the active ingredient in tea. However, the nicotine receptors and foam cell formation mechanisms should to be further clarified. Application level in the future, we wish to prove tea active ingredients attenuate the effect of smoking and reduce foam cell formation. Finally, we promote the advantages of tea drinking and it’s clinical applications.
Description:	碩士指導教授-何元順共同指導教授-吳志雄委員-潘敏雄委員-陳志榮委員-楊順發
Data Type:	thesis
Appears in Collections:	[ ] Dissertations/Theses

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