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    請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/54081


    題名: 探討DNA Primase 1 (Prim1)在癌幹細胞中與幹細胞特性基因之關聯性
    The Potential Stemness Characters of DNA Primase 1 (Prim1) Involved in Breast Cancer Stem-like Cells
    作者: 李欣怡
    Lee, Hsin-I
    關鍵詞: 乳癌;引子酶;癌幹細胞;breast cancer;Prim1;CSCs
    日期: 2012-07-03
    上傳時間: 2018-11-16 11:22:09 (UTC+8)
    摘要: 由於先前文獻利用DNA微陣列(Microarray analysis)偵測乳癌細胞株中相關基因的表現，發現Prim1在乳癌細胞株中大量表現；先前實驗室已探討Prim1與乳癌之間的關聯性，並未探討在癌幹細胞中與Prim1的關聯。在254個乳癌病患檢體中觀察到Prim1在乳癌病患的腫瘤組織中表現量高於正常組織，且在預後狀況以及腫瘤大小有顯著的差異，在細胞株當中證實將Prim1抑制其表現會影響細胞週期及細胞移行的能力。
    本研究初步分析Prim1在13株乳癌細胞株進行RT-PCR及西方點墨法分析，發現Prim1在乳癌細胞株中mRNNA及蛋白質表現相較正常乳腺細胞有高度表現，接著在13株乳癌細胞株當進行RT-PCR分析幹細胞的特性，並分析側群細胞(Side Population, SP)的比例，及分析細胞的CD44+/CD24-的表現建立乳癌幹細胞的分析方式。之後在穩定表現siPrim1的細胞中分析側群細胞與CD44/CD24的表現，發現在側群細胞或表現CD44+/CD24-的細胞的比例皆有下降，並觀察野生型MCF-7分選出CD44+/CD24-的細胞中Prim1的表現高於CD44+/CD24+的細胞，並利用RT-PCR分析發現Prim1與幹細胞特性基因(Sox2、Oct4、β-catenin及Notch1)有相關性。在siPrim1分選出CD44+/CD24-的細胞表現的腫瘤球體(Tumorsphere)明顯小於由野生型MCF7分選出CD44+/CD24-的細胞。接著利用天然萃取物Inotilone可抑制Prim1的表現且抑制野生型MCF7細胞的生長與側群細胞的比例；因此本篇研究推論Prim1與癌幹細胞可能具有相關性，並期望未來能夠使用天然萃取物降低癌幹細胞的比例以增加臨床治療的效果。

    The main purpose of this study was to explore the correlation with cancer stem-like cell (CSCs) and DNA Primase 1 (Prim1) in breast cancer. In recent years, multiple reporters have shown that subpopulation of so called cancer stem cells (CSCs; also called stem-like cell or tumor-initiating cells) are also acquired for sustained tumor growth and progression, and may be responsible for cancer recurrence and metastasis. Our lab previously used RT-PCR and Real-time PCR to do examination of thirteen breast cancer cells and 254 patient samples, and then we found PRIM1 have conspicuous expression in breast cancer and patient samples.Then we used siRNA to knockdown Prim1 expression in the breast cancer to see the mechanism of Prim1 can inhibit breast cancer in previous studies as well as Prim1 and want to screen some stemness marker in this study. And to identify some natural medicine can inhibit Prim1 expression in breast cancer, thus reducing the occurrence of breast cancer, hope the future more in-depth discussion the mechanism Prim1 in breast cancer and use natural medicine to improve the treatment or reduce the occurrence of rate in breast cancer. In the future, we hope to find out the important transcription factors that due to Nicotine or Estrogen stimulation to regulate Prim1 expression. In the future, we expect to be able to improve the treatment of breast cancer or reduce the incidence.
    描述: 碩士
    指導教授-何元順
    委員-林亮音
    委員-賴基銘
    資料類型: thesis
    顯示於類別:[醫學檢驗暨生物技術學系所] 博碩士論文

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