評估藻膠萃取物(CTX)在退化性關節炎之保護能力

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題名:	評估藻膠萃取物(CTX)在退化性關節炎之保護能力 Evaluation of the Protective Effects of the Alginate Extract(CTX) in Osteoarthritis
作者:	姚力凡 Yao, Li-Fan
貢獻者:	醫學檢驗暨生物技術學系陳建和鄭朝文
關鍵詞:	藻膠萃取物;退化性關節炎 Alginate Extract(CTX);Osteoarthritis
日期:	2012-06-27
上傳時間:	2018-11-16 10:04:24 (UTC+8)
摘要:	退化性關節炎（OA）是一種因關節軟骨遭受破壞而造成關節出現多種退化性的症狀。目前對於OA的研究主要著重於治療的藥物或日常生活補充的保健食品是否可達到抑制具有分解作用的細胞激素產生和減緩關節軟骨的降解。事實上，在稍早的研究中，即針對一項由植物中萃取的CTX在軟骨基質合成與刺激軟骨新生的潛在能力。因此，本篇論文研究的目的則是藉由體內試驗和體外試驗更進一步證明CTX對於早期的OA預防功效為何。在體內試驗方面，利用慢性關節軟骨磨損誘導手術（ACLT）之動物模式來模擬人類OA，經口服投以CTX後，以解剖病理方式來評估CTX對於股骨和脛骨之軟骨保護效果。在體外試驗方面，則使用細胞培養模式來評估CTX對於軟骨分解相關細胞激素第一型介白素β（IL-1β）存在下對於軟骨保護的相關機轉，並進一步分析其對基質金屬蛋白酶類（MMPs）、軟骨蛋白聚醣（aggrecan）等軟骨相關成份的調控情形。然而，在投以CTX與OA控制組比較下，結果顯示CTX能降低基質金屬蛋白酶類（尤其是MMP-13）基因表現外，更能促進軟骨蛋白聚醣基因表達。此外，CTX對於OA在鎮痛及病徵亦有減緩的趨勢。本篇研究顯示，CTX於劑量3㏄/天在動物模式體內試驗上，與OA控制組相比，OA所導致的關節僵硬及軟骨磨損均能獲得改善。總而言之，未來在於早期OA的預防上，CTX可以提供正向的協助。 Osteoarthritis (OA) is a multifactorial degenerative joint disease with obvious cartilage degradation. Present studies in OA are emphasizing on invention of therapeutic reagents that can inhibit catabolism cytokines and slow down cartilage degradation rather than OA prophylaxis. Herbaceous extracted CTX has represented potential ability to promote matrix anabolism and stimulated cartilage regeneration. Therefore, the purpose of this study is to further investigate its preventive effects in early OA stage and verify the underlying molecular mechanisms through in vivo and in vitro experiments. The surgical anterior cruciate ligament transsection (ACLT) rabbit model will be used to assess the degenerative changes on articular cartilage. By oral-given of CTX, macroscopic and microscopic changes of femur and tibia will be further evaluated. Cell culture model will be established to exam the matrix turnover effects in Interleukin-1β (IL-1β) treated condition and evaluate the chondroprotective mechanisms of CTX. The expression of matrix metalloproteinases and aggrecan will be examined in both RNA and protein levels. However, these changes were markedly in CTX treated groups compared with OA control. These results show CTX exhibited ability to decrease MMPs (esp. MMP-13) and expression promoted aggrecan gene. In addition, CTX exhibited both analgesic and OA-modifying activities in vivo experiments. The results obtained in this study suggest that CTX 3㏄/daily of continuous oral treatment led to lesser degrees of articular stiffness and histological cartilage damage compared with OA controls days after OA inducement. Together, these data may be a promising approach for OA treatment.
描述:	碩士指導教授-陳建和共同指導教授-鄭朝文委員-黃嘯谷委員-廖辰中委員-林詠峯
資料類型:	thesis
顯示於類別:	[醫學檢驗暨生物技術學系所] 博碩士論文

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