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    題名: FXYD2蛋白質在脊髓損傷動物之表現
    The expression of FXYD2 protein in spinal cord injury animals
    作者: 陳郁雯
    Chen, Yu-Wen
    關鍵詞: 脊髓損傷;星狀細胞;神經細胞;FXYD2;spinal cord injury;astrocyte;neuron;glial scar
    日期: 2013-01-25
    上傳時間: 2018-11-12 11:37:18 (UTC+8)
    摘要: 脊髓損傷(Spinal Cord Injury) 最常見是由車禍意外、從高處摔落或是運動傷害等所造成。受傷該處的神經細胞因直接受到撞擊而死亡，接著又因二次傷害（secondary injury）所引發的神經細胞死亡，因而造成脊髓的永久損傷，傷勢嚴重者常導致全身癱瘓或半身癱瘓。研究至今對於脊髓損傷仍然沒有有效的治療方式，因此研究脊髓損傷造成神經細胞死亡的相關細胞及分子機制是目前世界上重要的研究課題之一，而其研發成果也成為找尋治療脊髓損傷藥物的關鍵。過去的初步研究發現脊髓損傷會誘導Fxyd2基因的表現增加，另外研究也發現Fxyd2基因剔除鼠於脊髓損傷後其脊髓損傷的程度明顯會比正常基因型老鼠的脊髓損傷輕微。這些證據均顯示FXYD2可能在中樞神經系統之中對於神經的受損與修復扮演一定的功能與角色。因此，在本研究中我們將探討脊髓損傷是否會影響FXYD2蛋白質的展現。我們利用自製抗體來探討在大鼠脊髓損傷後其FXYD2蛋白的表現。我們的結果顯示FXYD2蛋白質在正常大鼠脊髓主要表現於神經細胞當中，然而在星狀細胞中其表現則不顯著。此外，脊髓損傷顯著降低FXYD2蛋白質的表現量。免疫螢光染色實驗進一步發現FXYD2蛋白質在脊髓損傷的動物於星狀細胞中的表現量明顯增加，而在去除Fxyd2基因的小鼠其glial scar (GFAP的免疫染色)比起正常基因型的小鼠來的少。以上的證據整體顯示FXYD2蛋白質似乎在脊髓受損後glial scar的形成扮演重要的角色。

    Spinal cord injury (SCI) results predominantly from traffic accidents, fall, sports accidents or violence. SCI includes the primary injury causing the direct death of neurons after the direct impact force and the secondary injury leading to the gradual loss of neurons from days to weeks after injury. Up to now, there is still no effective treatment for SCI patients. Thus, it is imperative to fully understand the cellular and molecular mechanisms underlying SCI before we can develop effective treatments or strategies for treating SCI patients. Our preliminary data showed that SCI up-regulated the expression of Fxyd2 mRNA. The Fxyd2 knockout mice showed a better locomotor activity than the wild-type littermates following spinal cord injury. The above findings suggest that FXYD2 may play an important role in neural repair. In this thesis, we investigated if spinal cord injury affected the expression of FXYD2 protein. We generated anti-rat FXYD2 antibody and used this antibody to detect the expression of FXYD2 protein using the Western blotting technique and the immunofluorescence technique. Our results showed that the spinal cord of mouse and rats expressed FXYD2-a?nprotein. We also found the FXYD2 protein was expressed mainly in the neurons of spinal cord; but barely detected in the astrocytes. Moreover, spinal cord injury decreased the expression of FXYD2 protein. Furthermore, the FXYD2 protein was increasingly expressed in the astrocytes of the spinal cord following injury. In addition, we found that Fxyd2 knockout mice had a smaller glial scar detected by GFAP staining than the wild-type control mice following SCI. Our findings together strongly suggest that FXYD2 protein may play an important role in the glial scar formation following injury.
    描述: 碩士
    指導教授-楊良友
    委員-陳彥州
    委員-林靜茹
    資料類型: thesis
    顯示於類別:[醫學科學研究所] 博碩士論文

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