Taipei Medical University Institutional Repository:Item 987654321/5337
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 45073/58249 (77%)
造访人次 : 2390045      在线人数 : 161
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/5337


    题名: 睪丸鋅指蛋白基因TZF-376之分子特性探討
    Molecular Characterization of Testis Zinc Finger Protein Gene—TZF376
    作者: 潘信全
    Shin-Chyuan Pan
    贡献者: 醫學研究所
    关键词: 鋅指蛋白
    表現序列標示
    原位雜交法
    testis zinc finger
    EST
    in situ hybridization
    日期: 2001
    上传时间: 2009-09-11 15:30:12 (UTC+8)
    摘要: 目前約有六分之一的夫婦可能面臨不孕症的困擾,而其中有
    一半問題是出自於男性。主要造成男性不孕的原因,又以在精子
    熟成(spermatogenesis)過程中發生障礙所造成的原因為最主要。
    已知有許多鋅指蛋白(zinc finger proteins)會在精子的發育過程
    表現,可能影響精子發育及其功能。鋅指蛋白是一種很常見的
    DNA結合蛋白,一般是以扮演轉錄因子(transcription factor)的
    角色,調節許多其它基因之表現。為了尋找精子熟成過程中新的
    鋅指蛋白,我們利用網路上的基因庫進行人類表現序列標示
    (expressing sequence tags)(簡稱EST)clones的搜尋,輸入關
    鍵字testis及zinc finger,找到一些表現在睪丸的鋅指蛋白類EST
    clones,並針對這些EST clones之序列來設計PCR引子,起
    初進行分析的EST clones有AI376558、AI003931、AI014681、
    AA417107四個,進行各個組織(例如:腦、肝、腎等組織)
    的反轉錄PCR(RT-PCR)實驗,以便瞭解此基因在各組織的表
    現,並製備riboprobes。結果發現AI376558在許多組織皆有表
    現,但是以睪丸表現量最高,此外在睪丸有兩個不同之PCR產
    物,我們猜測其有alternative splicing的情形,因此我們目前
    較專注於AI376558之進一步分析,並將其命名為TZF376。由定
    序結果發現這是一個具有四個C2H2鋅指區的鋅指蛋白,基因位
    在人類第一號染色體上。另外我們亦定序幾個相關的EST
    clones,結果顯示這個鋅指蛋白之alternative splicing相當地複
    雜,至少有四種不同的alternative splicing,其中有一個clone
    BE551217缺少一個20 bp之核酸片段,結果造成三個鋅指區之
    缺損,這個蛋白質有可能對具鋅指區之蛋白產生負調節作用。此
    外,我們根據第一號染色體上之基因組序列設計三組橫跨
    TZF376 exons之primers,以檢測180例不孕症之男性是否有
    TZF376基因之缺損情形。而更進一步原位雜交分析將有助於瞭
    解這個鋅指蛋白在睪丸中精細胞(germ cells)及體細胞(Sertoli
    and Leydig cells)的表現情形,如此可對基因之功能作一初步預
    測。
    About one-sixth of the couples have the problem with infertility
    nowadays, and half of the problems come from male. Most of male
    infertility results from the impairment of spermatogenesis. We searched
    EST Genebank and identified several testis zinc finger protein genes. We
    would like to know whether these genes could influence the development
    and function of testis. Zinc finger is an extremely common protein domain
    of DNA binding proteins, and zinc finger proteins usually regulate other
    gene expression via their roles as transcription factors. The sequences of
    these zinc finger EST clones was used to design the PCR primers. RT-PCR
    analysis indicated that one of the EST clones, AI376558 might have some
    alternative splicing processing. We now focus on this EST clone and name
    this gene TZF376. Sequence analysis of several related EST clones showed
    that the alternative splicing of TZF376 is complicated. We have found at
    least 5 different alternative spliced EST clones, BE551217, with a 20 nt
    deletion encodes a truncated protein without zinc finger domain. In situ
    hybridization analysis was performed to characterize the expression pattern
    of TZF376. The localization in germ cells, Sertoli or Leydig cells of testis
    will imply the function of the genes. Intensive PCR screening of the
    TZF376 coding region on human chromosome 1 were performed to see if
    any deletion occur in cellular DNA of 180 infertile patients.These results
    will imply the correlation between TZF376 gene infertility.
    数据类型: thesis
    显示于类别:[醫學科學研究所] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    摘要.doc37KbMicrosoft Word121检视/开启
    摘要.pdf69KbAdobe PDF141检视/开启
    摘要.ppt114KbMicrosoft Powerpoint107检视/开启
    摘要.ps423KbPostscript69检视/开启


    在TMUIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    著作權聲明 Copyright Notice
    • 本平台之數位內容為臺北醫學大學所收錄之機構典藏,包含體系內各式學術著作及學術產出。秉持開放取用的精神,提供使用者進行資料檢索、下載與取用,惟仍請適度、合理地於合法範圍內使用本平台之內容,以尊重著作權人之權益。商業上之利用,請先取得著作權人之授權。

      The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.

    • 瀏覽或使用本平台,視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例,並不得為任何不法目的使用TMUIR。

      By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.

    • 本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者,煩請權利人通知本平台維護人員([email protected]),將立即採取移除該數位著作等補救措施。

      TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

    Back to Top
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈