(1)嘗試以陽離子交換樹脂層析法純化豬精漿內已知的精子活動力抑制蛋 白SMI之研究; (2)維生素K3對人類子宮頸癌細胞之抗癌活性及作用機轉之 研究

TMUIR > College of Medicine > Graduate Institute of Medical Sciences > Dissertation/Thesis > Item 987654321/5209

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题名:	(1)嘗試以陽離子交換樹脂層析法純化豬精漿內已知的精子活動力抑制蛋白SMI之研究;(2)維生素K3對人類子宮頸癌細胞之抗癌活性及作用機轉之研究 (1)The Utilization of Cation Exchanger to Purify Sperm Motility Inhibitor (SMI) from Porcine Seminal Plasma;(2) The Anitumor Acitivy and Machanistic Study of Vitamin K3 against Human Cervical Carcino
作者:	孫德萍 Sun, Te-Ping
贡献者:	醫學研究所
关键词:	精子活動力抑制蛋白維生素K3 子宮頸癌細胞 SMI Vitamin K3 Cervical Carcinoma
日期:	1996
上传时间:	2009-09-11 15:26:25 (UTC+8)
摘要:	(一) 精漿 (seminal plasma) 中的物質主要由雄性副性腺的分泌物組成，如精囊 (seminal vesicle)，前列腺 (prostate gland) 以及尿道球腺 (bulbourethal gland) 的分泌物等。SMI (sperm motility inhibitor) 乃由豬精漿中分離純化，含91個胺基酸，具有抑制公豬精子活動力及降低其頭帽反應頻率的蛋白質。今另嘗試以陽離子交換樹脂層析法純化之。利用陽離子交換層析柱carboxymethyl cellulose chroma-tography (CM52) 分離豬精漿液中蛋白質，收集20 mM HEPES，pH 7.9，1 mM EDTA的緩衝沖洗液，再以陽離子交換層析柱phosphocellulose chromatography (P11) 吸附後，在含氯化鉀0~0.1 M之20 mM HEPES，pH 7.9，1 mM EDTA緩衝鹽溶液中，可沖提出SMI。純化的蛋白質經濃縮並去除鹽份後，即以SDS- PAGE，HPLC及質量質譜分析儀分析。以質量質譜分析儀分析此蛋白質質量為 (10,0671) dalton。證實豬精漿液在經過2次陽離子交換層析純化後，可獲得純化之SMI蛋白質。吾更進一步根據其於體內分布，選用肺炎雙球菌測試對其生長能力的影響，結果顯示並無抑制作用。 (二) 維生素 K3 (VK3，2-甲基-1,4- 奈昆 ) 是兩種天然維生素K1及K2的人工合成類似物。VK3對不同囓齒動物及人類腫瘤細胞具有廣泛之抑制生長作用。而且已知VK3是維生素K族中最強之抗癌藥。在美及臺已進行第一、二期之臨床試驗。以sulforhodamine B (SRB) 染色法得知VK3對人類子宮頸癌SiHa細胞株的半致死劑量為37微莫耳濃度。在作用機轉之研究中，發現VK3在 SiHa細胞中可引起： (1) 細胞週期運轉延遲於S或G2/M期，(2) 改變具細胞週期特異性蛋白質之表現或磷酸化之程度，如CDK1激活，Cdc25磷酸 ，Cyclins A及E。為了闡明VK3直接標的物，以人工合成一段十一個胺基酸胜鏈（含遍存於蛋白質酪胺酸磷酸 (protein tyrosine phosphatase) 及雙重特異性蛋白質磷酸 (dual specificity protein phosphatase) 活化中心）與帶有放射性之VK3作用證明，VK3可和此鏈內之cysteine (Cys) 胺基酸結合，使酵素失去活性，但無法與由Cys突變為 serine (Ser) 的胜鏈結合。VK3可能是目前臨床抗癌藥物中，第一個被發現為蛋白質酪胺酸磷酸的抑制劑。上述結果顯示 VK3 可能因與Cdc25 磷酸之Cys結合，使其活性散失進而使CDK1激活酵素處於高度磷酸化而失去活性，導致癌細胞無法進行細胞週期運轉而死亡。 (PART I): Seminal plasma has been shown to be composed of fluid from male accessory sex glands, such as seminal vesicle, prostate gland and bulbourethal gland etc. Sperm motility inhibitor (SMI) was previously purified from porcine seminal plasma as described and the utilization of cation exchanger to purify was also tried in this report. SMI was shown to contain 91 amino acids, and to decrease the sperm motility and its frequency of acrosomal reaction rate of spermatozoa. Carboxymethyl cellulose chromatography (CM52) was used to partial purify SMI protein from porcine seminal plasma. The fractions containing SMI protein were obtained in the flow through buffer (20 mM HEPES, pH 7.9 and 1mM EDTA; PC buffer) as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high performance liquid chromatography (RP-HPLC). After the SMI was checked and surely contained in the collected fractions, the phosphocellulose chromato-graphy was used for the further purification. And then the purified SMI protein was found in the gradient PC buffer containing 0 ~ 0.1 M KCl. The result was also checked by SDS-PAGE, RP-HPLC and mass spectrophotometer. The molecular weight of SMI determined by mass spectrophoto-meter is (10,0671) dalton, which is the same as previously reported by others.SMI was identical to b-microseminoprotein and was also found in the mucosa of respiratory and digestive tract. The Streptococcus pneumonia was chosen to clarify the role of SMI (2 and 20 mg) in the mucous secretory tissue. No inhibitory effect on the growth of Streptococcus pneumonia was found after 24 h incubation in a humidified 5 % CO2 incubator. (PART II): Vitamin K3 (VK3, 2-methyl-1,4-naphthoquinone) is the synthetic derivative of two naturally occuring vitamin K1 and K2. It has been shown that VK3 exhibits a broad spectrum of antitumor activity in rodent and human tumor cells. Among these three VK congeners, VK3 exerts the most toxic effect toward cancer cells. A phase I/II clinical trials of VK3 has been performed in both U.S. and Taiwan. The IC50 of VK3 against human cervical carcinoma SiHa cells is 37 mM as determined by sulforhodamine B (SRB) colorimetric assay. In the mechanistic study of VK3s action on SiHa cells, we found that VK3 induces: (1) the cell cycle arrest or delayed at the S or G2/M phase, (2) the alterations of the expression or phosphorylation status of cell cycle specific proteins, such as CDK1, Cdc25 phosphatase, Cyclins A and E. For the elucidation of the direct target of VK3s action on cells, the synthesized undecapeptide, containing the conserved cysteine (Cys) active domain of protein tyrosine phosphatases family and dual specificity protein phosphatases family, were incubated with 3H-VK3 in vitro. VK3 was found to bind directly to the peptide containing Cys residue but not to the mutant couenterpartner containing Cys mutated to serine (Ser). Among the clinically used anticancer drugs, VK3 might be the first drug that acts as an inhibitor of protein tyrosine phosphatases. Our results suggest that binding of VK3 to the Cys residue at the active site of Cdc25 phosphatase generates the hyperphosphorylated inactive form of CDK1 which in turn induces cell cycle arrest, leading to the cell death.
数据类型:	thesis
显示于类别:	[Graduate Institute of Medical Sciences] Dissertation/Thesis

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