於大鼠模式中探討重複創傷性腦損傷之神經行為變化

Taipei Medical University Institutional Repository > 藥學院 > 臨床藥物基因體學暨蛋白質體學碩士學位學程 > 博碩士論文 > Item 987654321/51596

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題名:	於大鼠模式中探討重複創傷性腦損傷之神經行為變化 The Studies of Neurobehavioral Changes after Repetitive Traumatic Brain Injury in Rats
作者:	洪國峯
關鍵詞:	腦創傷;動物模型;動物行為測試;traumatic brain injury;animal model;animal behavior test
日期:	2017-01-18
上傳時間:	2018-10-15 16:29:57 (UTC+8)
摘要:	創傷性腦損傷(Traumatic brain injury)在世界各地都是一項重要的公共衛生議題，尤其輕度腦損傷(Mild Traumatic brain injury, mTBI )患者，佔神經外傷人數高達80%，而其中又以重複輕度腦損傷(Repetitive mild traumatic brain injury, rmTBI)為主，研究指出有些患者腦部會產生過度磷酸化的tau蛋白、散佈型的星狀細胞及小神經膠質細胞增生和慢性神經元退化等慢性創傷腦病變(chronic traumatic encephalopathy, CTE)症狀，造成壽命減短及神經退化疾病的發生率上升，但是長久以來還是沒有客觀的診斷標準，進而造成無法有效診斷與治療。本實驗使用9週齡雄性Sprague-Dawley(SD)大鼠，隨機分成兩組，分別為控制組(僅吸入和撞擊組相同氣麻量)以及不同撞擊重量之重複輕度腦損傷組(給予重複相同程度的撞擊力道) (Repetitive mild traumatic brain injury)。之後藉由六項包括運動、感覺行為及認知測試，評估大鼠受傷程度與行為認知功能受損的關聯性，在結果上可以看到在不同嚴重程度的大鼠動物模型中發現，受傷越嚴重的動物對於平衡能力、神經學分數以及認知功能上的表現也越差。由於臨床收案，需要長時間的觀察與測試，才能明顯看出是否有行為及認知功能退化，本研究以實驗動物模型分析重複腦創傷後其受傷程度及行為、認知功能的關聯性。希望可以藉由建立好的動物模型來模擬人類腦部受傷後的情形，並且試著找出有效的診斷標的，進而提早預防以及治療。 Traumatic brain injury all over the world is an important public health issue, especially mild traumatic brain injury (mTBI). It is up to 80% in neurotrauma, especially repetitive mild traumatic brain injury (rmTBI) and another problem is chronic traumatic encephalopathy (CTE). Researchers pointed out that hyperphosphorylation of the tau protein, spreading astrocyte and microglial cell hyperplasia and chronic neuron degeneration etc. in some rmTBI patients the brain, that might reduce the lifespan and increase the neuron degenerative disease rate; however, diagnosis is still lacking and current treatment is also not very effective. In this studies, we used nine-week-old male Sprague-Dawley (SD) rats, which were randomly divided into two groups, as the control group (only Gas anesthesia treated) and the treatment group (rmTBI) by weight drop impact. Six tests were carried out for behavioral and cognitive tests to assess the degree of injury associated with the degradation of the behavioral and cognitive functions. In results, the gradations in impact pressure produced progressive degrees of injury severity in the balance function, neurological score as well as cognitive function. The test model of a rmTBI animal, which mimics the repetitive TBI patient’s injured conditions, may allow to follow the long-term observations and to develop an effective diagnosis, prevention, and treatment of behavioral and cognitive dysfunction.
描述:	碩士指導教授-楊維中共同指導教授-蔣永孝委員-謝宗勳委員-王家儀委員-李怡萱
資料類型:	thesis
顯示於類別:	[臨床藥物基因體學暨蛋白質體學碩士學位學程] 博碩士論文

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