Taipei Medical University Institutional Repository:Item 987654321/51438
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 45069/58245 (77%)
造访人次 : 2346703      在线人数 : 310
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/51438


    题名: 丙戊酸 (Valproic Acid)對於脊髓損傷大鼠之影響
    Influence of Valproic Acid on Spinal Cord Injury of Rats
    作者: 林紘帆
    Lin, Hong-Fan
    贡献者: 傷害防治學研究所
    洪國盛
    关键词: 脊髓損傷;Valproic acid;細胞凋亡;神經元
    Spianl cord injury;Valproic acid;apoptosis;neuron
    日期: 2012-06-24
    上传时间: 2018-10-12 10:05:43 (UTC+8)
    摘要: 前言：外傷性脊髓損傷 (Traumatic Spinal Cord Injury, SCI)所引發初期發炎反應會造成神經元受損、細胞型態變異、細胞凋亡，並誘發相關蛋白質量的增減。Valproic acid (VPA)為目前廣泛用於治療癲癇的藥物，已有研究顯示VPA為組織蛋白去乙醯化酶的抑制劑，並可調控基因的表現調節轉錄作用，且組織蛋白的乙醯化作用為細胞發炎以及修復過程的關鍵。
    方法：本實驗分成兩部份，第一部份為低劑量組 (30mg/kg,7d)並隨機分成四組—假手術組、假手術給予VPA治療組、脊髓損傷組、VPA治療組；第二部份為單一劑量組 (30mg/kg、100mg/kg、200mg/kg,24hr)分組方式與第一部份相同。使用Basso, Beattie and Bresnahan (BBB)評分表衡量四組大鼠的行為運動功能，利用免疫螢光染色探討神經元形態及細胞凋亡的情形以及西方墨點法下各蛋白質的表現量。
    結果： VPA會促進脊髓損傷後神經元的修復，降低細胞凋亡的發生，增加神經突觸的生長，並且有益於損傷後大鼠的行為運動功能。
    Introduction－In initial inflammatory reactions induced by Traumatic Spinal Cord Injury (SCI) causes damages to neurons, morphological changes at the cellular level, apoptosis, and a decrease in the expression of pertinent proteins. Valproic acid, a commonly used epilepsy treatment, has been shown to inhibit the deacetylation of histones, which is a critical step in the inflammatory and repair processes. In addition, VPA is also implicated in the modulation of gene expression.

    Method－This experiment consists of 2 parts. Part 1 is the low dosage group, which is subdivided into 4 groups randomly: sham, sham+VPA, SCI, and SCI+VPA. Part 2 is the single dosage group, which is subdivided into 4 groups in the same manner as group 1. The Basso, Beattie and Bresnahan (BBB) scoring method was then applied to analyze the locomotion of rats. Immunoflurecence was applied to analyze the morphological changes in neurons and apoptosis. Furthermore, western blot was used to quantify the expression of proteins.

    Result－VPA promoted the healing of neurons and the growth of axons after SCI. In addition, it also caused a decrease in apoptosis, and improved in the locomotion of rats.
    描述: 碩士
    委員-黃勝堅
    委員-黃銓珍
    指導教授-洪國盛
    数据类型: thesis
    显示于类别:[傷害防治學研究所] 碩博士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML360检视/开启


    检视Licence

    在TMUIR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    著作權聲明 Copyright Notice

    • 本平台之數位內容為臺北醫學大學所收錄之機構典藏，包含體系內各式學術著作及學術產出。秉持開放取用的精神，提供使用者進行資料檢索、下載與取用，惟仍請適度、合理地於合法範圍內使用本平台之內容，以尊重著作權人之權益。商業上之利用，請先取得著作權人之授權。

      The digital content on this platform is part of the Taipei Medical University Institutional Repository, featuring various academic works and outputs from the institution. It offers free access to academic research and public education for non-commercial use. Please use the content appropriately and within legal boundaries to respect copyright owners' rights. For commercial use, please obtain prior authorization from the copyright owner.

    • 瀏覽或使用本平台，視同使用者已完全接受並瞭解聲明中所有規範、中華民國相關法規、一切國際網路規定及使用慣例，並不得為任何不法目的使用TMUIR。

      By utilising the platform, users are deemed to have fully accepted and understood all the regulations set out in the statement, relevant laws of the Republic of China, all international internet regulations, and usage conventions. Furthermore, users must not use TMUIR for any illegal purposes.

    • 本平台盡力防止侵害著作權人之權益。若發現本平台之數位內容有侵害著作權人權益情事者，煩請權利人通知本平台維護人員([email protected])，將立即採取移除該數位著作等補救措施。

      TMUIR is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff([email protected]). We will remove the work from the repository.

    Back to Top
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈