麩醯胺對於肺部發炎反應免疫調節之影響

Taipei Medical University Institutional Repository > 營養學院 > 保健營養學系暨研究所 > 碩博士論文 > Item 987654321/51266

請使用永久網址來引用或連結此文件: http://libir.tmu.edu.tw/handle/987654321/51266

題名:	麩醯胺對於肺部發炎反應免疫調節之影響 Immunoregulatory effects of glutamine in pulmonary inflammation
作者:	侯又禎 Hou, Yu-Chen
貢獻者:	保健營養學研究所葉松鈴
關鍵詞:	麩醯胺;脂多醣;NF-κB;mTOR;急性肺損傷;發炎之緩解;調節型T細胞 glutamine;lipopolysaccharide;NF-κB;mTOR;acute lung injury;resolution of inflammation;Treg
日期:	2012-06-05
上傳時間:	2018-10-08 11:27:01 (UTC+8)
摘要:	免疫防禦能力於維持呼吸系統之健康扮演相當重要的角色，當病原菌入侵呼吸道時，會誘發呼吸道上皮細胞nuclear factor (NF)-κB之活化，促進CXC化學趨化因子生成，吸引嗜中性白血球聚集至發炎部位清除病源，而發炎之緩解則受到調節型T細胞(regulatory T cell, Treg)所調控。肺臟是維持體內麩醯胺(glutamine, GLN)恆定的重要器官，而GLN為臨床上常用之免疫調節營養素，本研究利用細胞實驗探討GLN對呼吸道上皮細胞NF-κB活化之影響，並利用急性肺損傷(acute lung injury, ALI)動物模式，探討GLN對於緩解肺部發炎反應之影響。研究結果發現，GLN透過Akt/mTOR/IKKβ之途徑，抑制lipopolysaccharide (LPS)誘發人類支氣管上皮細胞株BEAS-2B細胞NF-κB之活化。GLN降低NF-κB之轉錄能力，減少NF-κB目標基因的表現，而生理濃度GLN (0.5 mM)對NF-κB之抑制效果最好。動物實驗以LPS引致C57BL/6J小鼠肺損傷一天之後，每天灌食0.5 g GLN/kg BW，連續9天，給予GLN可增加肺泡灌洗液中Treg之比率，進而促進嗜中性白血球之清除；並抑制輔助型T細胞(helper T cells, Th cells)表現interleukin-17，緩解肺部之發炎反應。本研究結果顯示，GLN透過調節先天性和後天性免疫反應減緩呼吸道之發炎，可提供臨床上ALI病人使用GLN營養介入之參考。 Immune defense system plays a critical role in maintaining the health of respiratory functions. In the presence of microorganisms, airway epithelial cells provoke inflammatory responses mediated by nuclear factor (NF)-κB. Activation of NF-κB promotes production of CXC chemokines that leads to neutrophil recruitment to eliminate pathogens invaded into lungs. T helper (Th) cells modulate lung inflammatory reaction and regulatory T cells (Tregs) participate in the resolution of inflammation. The lungs are an important organ for glutamine (GLN) homeostasis and GLN is a nutrient commonly used in immunonutrition regimens. This study investigated the effects of GLN on activation of NF-κB in BEAS-2B cell line derived from human bronchial epithelial cells. Also, the possible roles of GLN on resolution of inflammation in a murine model of acute lung injury (ALI) were studied. The results showed that GLN modulated lipopolysaccharide (LPS)-induced activation of NF-κB through the Akt/mTOR/IKKβ pathway in BEAS-2B cells. GLN administration decreased the transcriptional activity of NF-κB, which suppressed the expression of NF-κB-targeted genes. Physiological concentration of GLN (0.5 mM) had a greater extent of inhibition on the NF-κB signaling cascade. In the animal study, LPS was instilled intratracheally 1 day after ALI. Then the C57BL/6J mice were gavaged with 0.5 g GLN/kg BW daily for subsequent 9 days. The results found that GLN resolved lung inflammation by increasing the population of Tregs in bronchoalveolar lavage fluid which promoted neutrophil clearance, and suppressed the expression of interleukin-17. This study indicated that GLN attenuated pulmonary inflammation at least partly by modulating both innate and adaptive immune responses. The findings imply that GLN can be considered as a nutritional supplement for ALI patients.
描述:	博士委員-林明燦委員-吳文惠委員-許瑞芬委員-陳彥州指導教授-葉松鈴
資料類型:	thesis
顯示於類別:	[保健營養學系暨研究所] 碩博士論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	143	檢視/開啟

檢視Licence

在TMUIR中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....