Study of ethnobotany and phytochemistry of Pavetta crassipes leaves and Calotropis procera bark from Malawi.

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題名:	Study of ethnobotany and phytochemistry of Pavetta crassipes leaves and Calotropis procera bark from Malawi.
作者:	約翰 Mponda John-Samson
關鍵詞:	槲皮素-3-O-芸香糖甙;綠原酸;甲基chlorogenate;Taraxasteryl 醋酸和Calactin;Pavetta crassipes;Calotropis procera;Malawi;Quercetin-3-O-rutinoside;Chlorogenic acid;Methyl chlorogenate;Taraxasteryl acetate and Calactin
日期:	2012-07-13
上傳時間:	2018-10-05 11:50:08 (UTC+8)
摘要:	This study aims to analyse the phytochemistry of two medicinal plants locally used by herbalists Pavetta crassipes leaves and Calotropis procera bark from Malawi. The specific objectives of the study are: To extract Pavetta crassipes leaves and Calotropis procera bark using 80% methanol and 100% methanol respectively. To isolate compounds using open column and medium pressure liquid chromatography. To elucidate the structures of isolated pure compounds using nuclear magnetic resonance spectral techniques. The research design was an experimental method. The dried Pavetta crassipes leaves (2900g) and Calotropis procera bark (4500g) was extracted with 3L 80% methanol and 100% MeOH respectively, at room temperature after 72 hours. Using various column chromatographical techniques each of the extracts was isolated to get the pure compounds. The isolated pure compounds were elucidated and characterized using Proton Nuclear magnetic Resonance Spectrum (1H NMR), Carbon Nuclear Magnetic Resonance (13C NMR), Heteronuclear Multiple Bond Coherence (HMBC), Heteronuclear Single Quantum Coherence (HSQC), Distortionless Enhancement via Polarization Transfer (DEPT), Nuclear Overhauser Effect Spectroscopy (NOESY) and Correlation Spectroscopy (COSY). The elucidation of pure compounds from Pavetta classipes yielded flavonoid Quercetin-3-O-rutinoside (1), Chlorogenic acid (2) and Methyl chlorogenate (3) and Calotropis procera yielded taraxasteryl acetate (4) and calactin (5).
描述:	碩士委員-鄭瑞棠委員-張永勳委員-李安榮委員-李美賢指導教授-徐鳳麟
資料類型:	thesis
顯示於類別:	[生藥學研究所] 博碩士論文

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