| 摘要: | 摘要
何首烏是中醫常用藥物，生品可潤腸通便、炮製品可補益精血；所
需藥材大多自大陸地區進口生乾何首烏塊根，炮製後再配銷於中藥房及
診所，然炮製品質至今仍無規範。因此本研究將先確定影響炮製結果的
因子。
研究方法共分為兩部分。第一部分先了解市售何首烏的品質，因此，
檢測何首烏市售品，包括台灣、大陸、台灣中藥廠材料，分析指標成分(2，
3，5，4'-tetrahydroxy -stilbene-2-O-β-D-glucoside，emodin and physcion)
的含量；結果顯示：台灣市售何首烏中炮製品比例甚高(33/35)；THSG 含
量大於1%：大陸樣品7 件有6 件，台灣中藥廠材料5 件有3 件，台灣市
售品35 件有1 件；emodin 及physcion 含量，台灣中藥廠與中區則高於
大陸。顯示兩岸對何首烏的品質認知不同。
第二部分自大陸地區進口之何首烏生乾塊根，用正交實驗設計，探
討浸潤液、浸潤溫度、浸潤時間、籠蒸時間等因子，對生乾何首烏炮製
的總多酚含量、消除DPPH 自由基、細胞生長刺激及粗多醣等指標的影
響，由統計極差結果，籠蒸時間是總多酚含量、消除DPPH 自由基、細
胞生長刺激最大影響因子，浸潤時間則是影響粗多醣最大因子。
確定輔料液體對新鮮何首烏的影響，以清水、稀黑豆汁液、濃黑豆
汁液浸潤冷凍乾燥的何首烏，結果顯示：清水浸潤有最低之消除DPPH
自由基的IC50。
Polygonum multiflorum (PM) was commonly prescribed in traditional Chinese medicine as laxative in raw type, while processed products was used as blood tonic. Most of PM requirement were fulfilled by importation of the dry RPM from mainland China. After them processed,they were distributed to pharmacy and clinic. Up to now, no guideline of processing has been available; therefore, the quality is much concerned by relevant profession.
The study was focused on the impact factors to affect the quality of processing RPM. Two parts were in the study. First part, we want to know the quality of marketed RPM so as to examine marker components of the sample (2,3,5,4'-tetrahydroxy-stilbene-2-O-β-D-glucoside (THSG),emodin andphyscion) from Taiwan,mainland China, and local pharmaceutical factories by HPLC method.
The outcomerevealed that content of THSG is over 1%; 6 out of 7 from mainland, 3 out of 5 local factories, only 1out of 35 from local market. However,Content of local subjects of factories and central areahad more emodin and physcion than from mainland Chain. Obviously,the quality of PM was different between strait.
Second part of study, was to researchthe impact factors of processing raw and dry PM imported from mainland with the orthogonal experimental design,which included soaking liquid,soaking temperature, soaking duration and steaming duration.
The outcomes of research reveal that DPPH radicals
scavenging,stimulation of cell growth and content of total polyphenol were impacted by the steaming duration while soaking duration would affect polysaccharide content at the first place.
The conclusion of orthogonal experiment indicated the antioxidative effects of RPM will be affected by increasing the time of steaming. The best processing
method is without any adjuvant material and other steaming factor for antioxidation. The concentration of adjuvant material will be considered for increasing the polysaccharide content. |
