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| 題名: | 單核球類幹細胞於皮膚修復之應用
Monocyte-derived stem-like cells as a new therapeutic approach for skin regeneration |
| 作者: | 江育旻
Chiang, Yu-Ming |
| 貢獻者: | 鄧文炳 |
| 關鍵詞: | 單核球;單核球類幹細胞;皮膚修復
Monocyte;Monocyte-derived stem cells;Skin rejuvenation |
| 日期: | 2013-06-19 |
| 上傳時間: | 2018-10-04 15:33:35 (UTC+8) |
| 摘要: | 本實驗研究目的是探討單核球類幹細胞對皮膚再生之影響。第一階段為建立單核球類幹細胞之培養系統。將老鼠之周邊血經磁珠分離法得到高度專一性的單核球細胞並經細胞膜表面抗原及分化定性確認後，再培養至含有纖維連接蛋白(Fibronectin)之培養皿，並以幹細胞相關基因、群落形成率及中胚層分化細胞監測得該細胞特性。過去文獻指出，幹細胞及其培養基均能有效促進皮膚再生之修復，其中轉化生長因子-β1（Transforming Growth Factor-β1, TGFβ1）為主要調控之生長因子，並於本實驗結果發現我們所分離出之單核球類幹細胞具有相當強之TGFβ1表現。鑑此，本研究第二階段建立單核球類幹細胞及其培養基於皮膚再生之修復模式。此階段實驗共分成美白、傷口修復及抗皺紋三方面進行。本實驗結果證實：在美白方面：單核球類幹細胞能減少黑色素含量，同時在黑色素相關基因表現上能抑制黑色素生成之下游基因TRP-1(Tyrosinase related protein 1)表現；在傷口修復上，單核球類幹細胞之培養基經量化評分傷口修復程度後證實能降低發炎反應促進傷口的癒合；抗皺方面，注射過單核球類幹細胞培養基的老化老鼠經Masson染色後顯示膠原蛋白含量增加，皺褶與對照組相對較少。藉由此初步平台的建立，未來可再經長期研究單核球類幹細胞對於不同疾病模式的動物的影響，並分析單核球類幹細胞之細胞分泌激素，建立老鼠至人類的單核球之研究應用平台，最終利用於人類之臨床治療上。
Monocytes are known to originate in the bone marrow and peripheral blood. They generally leave the blood and move into tissue, and differentiate into tissue resident macrophages and dendritic cells. Here we reported the Ly6C+ monocytes isolated from murine peripheral blood, and then cultured on fibronectin-coated plate that could significantly increase of stem cell related gene expression, such as CD73, CD105, type I collagen, fibronectin , and surface markers phenotype(CD44, CD45,CD34, CD105). The circulating monocytes also had capabilities of self-renew and differentiation into several mesenchymal cell lineages. We named this cell population as the monocyte-derived stem cell (MDSC). Based on recent studies, they indicated that peripheral blood monocular cells might improve skin regeneration; we analyzed the effect of MDSC or MDSC-CM (MDSC conditional medium) in three aspects of skin rejuvenation in our study; included whitening, wound healing and anti-wrinkling. Our results showed that MDSC-CM might reduce melanin content of melanoma and inhibit the tyrosinase related protein 1(TRP-1) expression, may lead to impact on the melanin synthesis pathway. It promoted wound healing process in our animal model, and reduced collagen impairment to improve winkle development by masson trichrome stain. In summary, according to our research we might be able to use MDSC in application of diseases therapy. In future studies, we may analyze the cytokine contents of MDSC-CM and establish the experimental platform between murine and human to the clinical therapeutics. |
| 描述: | 碩士
指導教授-鄧文炳
委員-張文昌
委員-張明熙 |
| 資料類型: | thesis |
| 顯示於類別: | [生醫材料暨組織工程研究所] 博碩士論文
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