高電場抗病原菌汙染-非熱電漿於生物醫學應用研究

Taipei Medical University Institutional Repository > 口腔醫學院 > 生醫材料暨組織工程研究所 > 博碩士論文 > Item 987654321/50835

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題名:	高電場抗病原菌汙染-非熱電漿於生物醫學應用研究 Research on Pathogenic Bacteria Decontamination by High Electric Field - Nonthermal Plasma for Biomedical Applications
作者:	楊庫巴 Yankuba B. Manga
貢獻者:	歐耿良
關鍵詞:	綠色科技;高電場;非熱電漿;操作靈活性;醫學電漿技術;殺菌 green technology;high electric field;nonthermal plasma;operational flexibility;plasma medicine;sterilization
日期:	2013-01-26
上傳時間:	2018-10-03 17:54:48 (UTC+8)
摘要:	綜合許多研究已知非熱電漿處理有其一定程度的操作靈活性，近期電漿技術在醫學界亦引起了極大的關注並投入許多相關的研究。然而到目前為止，如欲完全滿足工業應用的需求目標仍有其技術上的限制。現今多提倡以綠色環保技術為中心目標，研究利用非熱電漿體其對環境的所產生殺菌效果，繼而以規劃發展新的滅菌研究。在此研究中，觀察通過平行金屬所產生的高電場，其針對金黃色葡萄球菌和大腸桿菌（E. coli）的滅菌效果。記錄影響細胞成長的因子以及分析有關其生長原理的官能基改變，利用以log10作為菌落形成的單位（CFU）來控制和處理樣品。這些細菌的試驗菌株的製備和稀釋皆符合日本工業標準JIS Z 2801:2010裡的抗菌率分析規範。將細菌樣品放入誘電體層玻璃並插入電極板之間，分別於2 kV和4 kV特的電力環境下，產生高電場等離子體的效果會在幾秒鐘內確定。以AC / DC電漿模型來驗證此種電漿離體系統中所需產生的電場，並使用電腦模擬來模擬電漿體模型。結果證明，產生的高電場1.7 x 106 ~ 2.7. x 106 (V/m)，能滅除99％和83％的金黃色葡萄球菌和大腸埃希氏菌（2.5×105～1.0×106個細胞ML-1），另時間設定為60秒內，比較2 kV的對照組和4 kV的實驗組，結果呈現將可於30秒的時間點上完成99％的細菌樣本滅菌。本研究提供針對高電場對微生物存活率的作用，並在固定時間點內完成了有效的滅菌。最後，本研究可從子顯微鏡（SEM 和TEM），穿透式電子顯微鏡和原子力顯微鏡（AFM）的照片，以及CAS的電場分析，便於理解其中的機制。可知此系統並未明顯的顯示出放電會對細菌結構造成損傷。若能確認並建立本系統將是可付諸實現的，未來即可廣泛的應用於牙科、醫療設備儀器、或水處理與空氣清淨等系統。 Nonthermal plasmas have tremendously attracted great attention and enormous research in vast field of plasma medicine due to their operational flexibility. But yet still, But yet still, their targets to fulfill the functionality of industrial applications are limited. Green technology as our central goal, investigation on efficacy of nonthermal plasma on bacterial sterilization to its environment led to new development and search route of bacterial sterilization mechanism. In this study, sterilization of staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) by generating high electric field by parallel metal plate was investigated. The effect of cells eruption and principle component analysis are used to identify functional group changes of 〖"Log" 〗_"10" number of colony forming units (CFU) on control and treated samples. These bacterial test strains are prepared and diluted in conformity with Japanese Industrial Standard JIS Z 2801:2010 for antibacterial rate analysis. Drop of this bacteria sample held by dielectric glass is inserted between electrode plates. Two different phenomena are carryout to produce a power supply of 2 kV and 4 kV. The effect of generating high electric field plasma is determined within seconds. Compliment using Computer Aided Simulation to simulate this plasma model was generated by AC/DC plasma model to validate required generated electric field and potential in this plasma system. The results shows that, the generated high electric field 1.7 x 106 ~ 2.7. x 106 (V/m) respectively, was able to deactivate 99% and 83% for staphylococcus aureus and Escherichia coli (2.5 x 105 ~ 1.0 x 106 cells ml-1) respectively within 60 sec against its control group for 2 kV and a complete sterilization is acquired for both bacterial samples of 99% at 30 sec for 4 kV power supply. This study provides an in depth inactivation mechanism of high electric field and the survival rate of these microbes shows an effective sterilization seconds. Finally, this mechanism is better understood from the capture electron microscopy (SEM & TEM) and atomic force microscope (AFM) images, and electric field analysis by CAS. Our system shows no apparent discharges and induced structural damage to these bacteria. This system is promising and if well establish could be widely applicable in areas like dentistry, less complicated medical devices, water treatment and air cleaning.
描述:	碩士指導教授-歐耿良委員-劉沖明委員-張榮善
資料類型:	thesis
顯示於類別:	[生醫材料暨組織工程研究所] 博碩士論文

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